论文部分内容阅读
目的环氧化酶-2基因(COX-2)的表达水平与临床病理特征有相关性,多态性可影响其表达水平,本文探讨COX-2的两个功能性单核甘酸多态(single nucleotide polymorphisms,SNPs)与膀胱癌易感性的关系。方法研究对象为250例经组织学确诊的膀胱癌病例和275例相匹配的无肿瘤史的正常人群。采用聚合酶链反应-限性片段长度多态性方法(PCR-RFCP)测COX-2基因-1195G/A和-765G>C多态的基因型,比较不同基因型与膀胱癌的关系,并分析其与分期、分级、吸烟之间的关系。结果 COX-2基因1195GG、GA和AA基因型在病例组和对照组间的频率分布有统计学意义(P<0.05),变量Logistic回归分析显示-1195AA基因型与膀胱癌发生的风险升高相关,OR值分别为(SNP-1195 GA+AA:OR=2.15,95%CI=1.25~3.70),含有A等位基因的GA+AA基因型相对于GG基因型,患者年龄、肿瘤分化程度、TNM分期及吸烟差异有统计学意义(P<0.05)。结论 COX-2基因启动子区的-1195G>A单核苷酸多态与可能膀胱癌遗传易感性相关,且可能与膀胱癌的肿瘤分化和TNM分期有关。
The expression of cyclooxygenase-2 gene (COX-2) is correlated with the clinicopathological features. Polymorphism may influence the expression level of cyclooxygenase-2 gene (COX-2) nucleotide polymorphisms, SNPs) and bladder cancer susceptibility. Methods The subjects were 250 histologically confirmed cases of bladder cancer and 275 matched normal controls without tumor history. The genotypes of -1195G / A and-765G> C polymorphisms of COX-2 gene were detected by polymerase chain reaction-restriction fragment length polymorphism (PCR-RFCP) and the relationships between different genotypes and bladder cancer were compared Analysis of its relationship with staging, grading, smoking. Results The frequency distribution of 1195GG, GA and AA genotypes in COX-2 gene was statistically significant between cases and controls (P <0.05). Logistic regression analysis showed that genotype-1195AA was associated with increased risk of bladder cancer (OR = 2.15, 95% CI = 1.25-3.70, respectively). The GA + AA genotypes with A allele were significantly higher than those with GG genotype, patient age, tumor differentiation, TNM staging and smoking differences were statistically significant (P <0.05). Conclusion The -1195G> A single nucleotide polymorphism in COX-2 gene promoter region may be related to the genetic susceptibility to bladder cancer and may be related to tumor differentiation and TNM staging in bladder cancer.