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试验以柠檬的嫩茎尖作外植体，通过组培育苗，培养出完整的柠檬瓶苗并移栽成活。最佳培养基分别为：改良ＭＳ＋６－ＢＡａ＋ＩＢＡｃ诱导茎尖；改良ＭＳ＋６－ＢＡｃ＋ＩＢＡａ培养不定芽增殖；改良ＭＳ＋ＩＢＡｃ＋ＡＢＴａ诱导生根。初次培养采用全暗条件；诱导、增殖阶段适宜温度２５±５℃；生根阶段适宜温度２０±５℃；培养基ｐＨ值均约５．８。结果表明：一个茎尖年繁殖系数为３１２个，移栽成活率达９０％以上。
The test uses the tip of the lemon tip as explants to cultivate a complete lemon seedling through tissue culture and transplanting. The best culture medium was: MS + 6-BAa + IBAc-induced shoot tip; MS + 6-BAc + IBAa-treated adventitious buds were proliferated; MS + IBAc + ABTa was used to induce rooting. Under the conditions of total darkness, the optimum temperature for induction and propagation was 25 ± 5 ℃, the suitable temperature for rooting was 20 ± 5 ℃, and the pH of medium was about 5.8. The results showed that the annual reproductive coefficient of one shoot was 312 and the survival rate of transplanting was more than 90%.