目的：建立测定细胞色素 Ｃ Ｙ Ｐ２ Ｄ６ 酶活性的毛细管气相色谱方法。方法：受试者口服右美沙芬（ Ｄ Ｍ） 后收集０ ～８ ｈ 尿样，经提取分离，以 Ｈ Ｐ１ 毛细管柱分离， Ｆ Ｉ Ｄ 为检测器。结果：右美沙芬与代谢物３羟基 Ｎ甲基吗喃（ Ｄ Ｔ） 分离良好， Ｄ Ｍ 在０２０ ～１６０ μｇ·ｍ Ｌ－ １ ， Ｄ Ｔ 在０４０ ～２０００ μｇ·ｍ Ｌ－ １ 范围内线性关系良好，回收率分别为９９５ ％ 和９９８ ％ ，日内相对标准偏差分别小于５４７ ％ 和６１９ ％ ， Ｄ Ｍ 和 Ｄ Ｔ 的最低检测限分别为８ ｎｇ 和４ ｎｇ 。用此法对１１ 名健康志愿者进行了 Ｃ Ｙ Ｐ２ Ｄ６ 活性测定。结论：此法简便、准确，可用于 Ｃ Ｙ Ｐ２ Ｄ６ 酶活性的测定。 Objective: To establish a capillary gas chromatographic method for the determination of cytochrome C Y P2 D6 enzyme activity. Methods: Subjects received dextromethorphan (D M) and collected urine samples for 0 to 8 hours. The samples were separated and separated by H P1 capillary column. F I D was the detector. Results: Dextromethorphan and metabolites 3hydroxynmethylmorphophthalocyanine (D T) were separated well with D M at 020 ~ 160 μg · m L -1 and D T at 040 ~ The linear range was 2000 μg · m L -1. The recoveries were 995% and 998%, respectively. The relative standard deviations were less than 547% and 619%, respectively. D M and D T The minimum detection limits were 8 ng and 4 ng, respectively. Eleven healthy volunteers were tested for C Y P2 D6 activity by this method. Conclusion: This method is simple, accurate and can be used for the determination of C Y P2 D6 enzyme activity.