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目的 探讨甲基毒死蜱在大鼠体内的代谢转化机制。方法 应用体外代谢系统对甲基毒死蜱的代谢机制进行研究 ,蛋白含量按Lowry法测定 ,甲基毒死蜱含量采用气相色谱法测定 ,甲基毒死蜱代谢产物的分离采用高效液相色谱法。结果 甲基毒死蜱主要是在机体的肝脏被代谢转化 ,肝细胞的微粒体和可溶性胞浆是主要的代谢转化场所。对甲基毒死蜱的代谢产物进行分离发现 ,在肝微粒体中存在明显的氧化代谢作用 ,微粒体和可溶性胞浆对甲基毒死蜱的代谢存在着不完全相同的生物转化系统。结论 甲基毒死蜱的主要代谢场所是肝细胞的微粒体和可溶性胞浆 ,氧化代谢是其主要的代谢方式
Objective To investigate the metabolic mechanism of methyl chlorpyrifos in rats. Methods The metabolic mechanism of methyl chlorpyrifos was studied by in vitro metabolic system. The content of chlorpyrifos was determined by Lowry method, the content of chlorpyrifos-methyl was determined by gas chromatography and the metabolite of chlorpyrifos-methyl was separated by high performance liquid chromatography. Results Methylchlorpyrifos was mainly metabolized and transformed in the liver of the body, and the microsomes and soluble cytoplasm of hepatocytes were the main sites of metabolic transformation. Metabolites of chlorpyrifos were isolated and found to have obvious oxidative metabolism in liver microsomes. Microsomes and soluble cytoplasm had different bioconversion systems to the metabolism of methyl chlorpyrifos. Conclusion The main metabolic sites of methyl chlorpyrifos are microsomes and soluble cytoplasm of hepatocytes. Oxidative metabolism is the main metabolic pathway