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目的:研究补肾化浊方对高脂饲料喂养大鼠肝功、血脂、血糖、胰岛素抵抗、瘦素的影响,探讨补肾化浊方防治非酒精性脂肪肝作用机制。方法:45只Wistar大鼠随机分为空白组、模型组、水林佳(19 g.kg-1)组和补肾化浊方(含生药20 g.kg-1)组。空白组予普通饲料喂养,其余3组予高脂饲料喂养,水林佳组、补肾化浊方组在高脂饲料喂养的同时进行相应的药物干预,12周后,处死大鼠,计算大鼠肝指数、Lee’s指数、胰岛素抵抗指数(IRI)、胰岛素敏感指数(ISI),检查肝功能、血脂、血糖、胰岛素(INS)、瘦素(LEP),并进行肝脏病理检查。结果:与模型组比较,补肾化浊方组、水林佳组均能明显改善大鼠丙氨酸氨基转移酶(ALT)、天门冬氨酸氨基转移酶(AST)、LEP水平(P<0.01);在改善大鼠INS,IRI,ISI方面,补肾化浊方组优于水林佳组(P<0.05或P<0.01),水林佳组对INS,IRI无明显改善作用,水林佳组、补肾化浊方组大鼠脂肪染色、糖原染色改善优于模型组(P<0.05或P<0.01)。结论:补肾化浊方通过保肝、调节血脂、改善瘦素及胰岛素抵抗等方面发挥治疗作用。
Objective: To study the effect of Bushen Huazhuo Fang on liver function, blood lipid, blood glucose, insulin resistance and leptin in rats fed with high-fat diet and explore the mechanism of Bushen Huazhuo Fang in preventing and treating non-alcoholic fatty liver. Methods: Forty-five Wistar rats were randomly divided into blank group, model group, Shui Lin Jia (19 g.kg-1) group and Bushen Huazhuo Fang (including crude drug 20 g.kg-1) group. The blank group was fed with normal feed and the other three groups were fed with high fat diet. The rats in Shuilin group and Bushen Huazhuo prescription group were fed with high fat diet and the corresponding drugs were intervened. After 12 weeks, Liver index, Lee’s index, insulin resistance index (IRI), insulin sensitivity index (ISI), liver function, blood lipids, blood glucose, insulin and leptin were measured. Liver pathology was also performed. Results: Compared with the model group, Bushen Huazhuo prescription group and Shuilinjia group could significantly improve the levels of ALT, AST and LEP (P <0.01) (P <0.05 or P <0.01), while Shuilinjia group had no significant effect on INS and IRI, while Shuilinjiaotong group was superior to Shuilinjiaji group in improving INS, IRI and ISI in rats. Group, Bushen Huazhuo prescription group, fat staining, glycogen staining better than the model group (P <0.05 or P <0.01). Conclusion: Bushen Huazhuo Fang has a therapeutic effect in protecting liver, regulating blood fat, improving leptin and insulin resistance.