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通过染色体工程将外源种质向小麦基因组转移的过程中,可以诱发受体物种基因组结构及基因表达的广泛遗传变异.本文以3个高代分离的小麦-黑麦姊妹易位系及其农艺亲本为材料,应用GISH和AFLP技术分析其基因组结构与组成,发现姊妹系材料基因组组成高度一致;同其亲本相比较,除1RS/1BL染色体易位外,并没有表现出其他明显的基因组结构变异.进一步的MSAP分析发现,易位系材料发生全甲基化修饰的比例比小麦亲本(全甲基化,16.37%;半甲基化,25.44%)明显上升(CN12,20.15%;CN17,20.91%;CN18,22.42%),而半甲基化比例则明显下降(CN12,21.41%;CN17,23.43%;CN18,22.42%).本研究共检测到29种不同类型的甲基化修饰模式,其中13种类型(33.74%)的位点表现为超甲基化修饰,9种类型(22.76%)的位点表现为去甲基化修饰,而余下7种类型(4.07%)的位点甲基化模式变异未能明确界定.从中分离了多条存在甲基化位点变异的DNA序列,鉴定了多种小麦转座子序列、亚端粒重复序列以及单拷贝蛋白质编码序列.
In the process of transferring exogenous germplasm to the genome of wheat by chromosomal engineering, it can induce a wide range of genetic variation in the genomic structure and gene expression of the recipient species.In this paper, three high-generation wheat-rye sister translocation lines and their agronomic Parents were used as materials. The genome structure and composition of Gossypium sibiricus were analyzed by GISH and AFLP. The results showed that the sibling lines were highly consistent in genome composition. Compared with their parents, other obvious genomic structural variations were not found except 1RS / 1BL chromosomal translocation . Further MSAP analysis showed that the percentage of full-methylation of metathesized material was significantly higher than that of wheat parents (full methylation, 16.37%; half methylation, 25.44%) (CN12, 20.15%; CN17, 20.91 %; CN18, 22.42%), while the proportion of hemimethylation was significantly decreased (CN12, 21.41%; CN17,23.43%; CN18,22.42%) In this study, 29 different types of methylation modification patterns were detected, Thirteen types (33.74%) showed hypermethylation, nine (22.76%) showed demethylation, while the remaining seven (4.07%) Baseline mutation was not clearly defined, and multiple methylated sites were isolated Heterologous DNA sequence, identified a number of transposon sequences in wheat, subtelomeric repeat protein coding sequence and a single copy.