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目的 探讨体外受精过程中胚胎污染的发生率、污染菌种和污染的来源。方法 回顾性分析 1999年 1月至 2 0 0 3年 6月 ,2 174个体外受精 胚胎移植 (IVF ET)周期中胚胎污染的发生率及污染菌种 ;随机抽取IVF ET中 6 1例精液分析正常男性的精液和 34例卵泡液进行细菌培养。结果发生胚胎污染 11例 ,发生率为 0 5 1% (11/ 2 174 ) ;污染菌种主要为大肠埃希菌 (4例 )和真菌 (4例 )。精液原液、处理后的精液上清液、精液原液与培养液的混合液及卵泡液细菌培养的阳性率分别为97% (2 9/ 30 )、10 % (3/ 30 )、6 % (2 / 31)及 9% (3/ 34)。结论 在体外受精的过程中 ,可发生部分胚胎污染 ,污染的菌种主要为大肠埃希菌和真菌。精液中的细菌可能是胚胎污染的主要来源。
Objective To investigate the incidence of embryo contamination during in vitro fertilization and the sources of contamination and contamination. Methods A retrospective analysis of the incidence of embryo contamination and contamination of 2 174 IVF ET cycles from January 1999 to June 2003 was performed. A total of 61 seminal fluid samples of IVF ET were randomly selected Normal male semen and 34 follicular fluid were cultured. Results There were 11 cases of embryo contamination, the incidence rate was 0 5 1% (11/2 174). The main types of contamination were Escherichia coli (4 cases) and fungi (4 cases). The positive rates of seminal fluid, treated semen supernatant, mixture of seminal fluid and culture medium and bacterial culture of follicular fluid were 97% (2/30), 10% (3/30), 6% (2) / 31) and 9% (3/34). Conclusion Some embryo contamination may occur in in vitro fertilization process. The main pollutants are Escherichia coli and fungi. Bacteria in semen may be the main source of embryo contamination.