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目的:检测合体滋养细胞微粒(STBM)对人脐静脉内皮细胞(HUVECs)增殖和凋亡的影响,探讨子痫前期可能的病因和发病机制。方法:选取2009年6月至10月在上海交通大学附属第六人民医院住院行选择性剖宫产的4例正常健康孕妇的胎盘,机械法体外制备STBM。STBM与HUVECs共培养,MTT法和流式细胞仪检测HUVECs的增殖和凋亡情况。结果:(1)STBM作用12h后,HUVECs悬浮细胞数增加,48h后可见大量悬浮细胞,细胞生存受到明显抑制;(2)30、90、150mg/L STBM作用48h后,内皮细胞增殖抑制率分别为(55.58±2.40)%、(67.94±1.93)%、(86.37±1.59)%,组间差异显著(P<0.01),呈剂量依赖性。(2)30、90、150mg/L STBM作用24h后,内皮细胞凋亡率分别为(17.39±1.11)%、(22.13±1.02)%、(30.42±1.98)%,组间差异显著(P<0.01),呈剂量依赖性。结论:STBM可抑制HUVECs细胞增殖,诱导细胞凋亡,提示其可能是子痫前期内皮细胞功能紊乱的原因。
Objective: To investigate the effect of syncytiotrophoblast (STBM) on the proliferation and apoptosis of human umbilical vein endothelial cells (HUVECs) and to explore the possible etiology and pathogenesis of preeclampsia. Methods: The placenta of 4 normal healthy pregnant women with selective cesarean section in the sixth affiliated hospital of Shanghai Jiao Tong University from June 2009 to October were selected and mechanically treated with STBM. STBM and HUVECs were co-cultured. The proliferation and apoptosis of HUVECs were detected by MTT assay and flow cytometry. Results: (1) The number of suspension cells increased after treated with STBM for 12h, and a large amount of suspension cells could be seen after 48h, and the cell survival was obviously inhibited. (2) The inhibitory rates of endothelial cell proliferation after treated with 30, 90 and 150mg / L STBM for 48h (55.58 ± 2.40)%, (67.94 ± 1.93)% and (86.37 ± 1.59)%, respectively. There was significant difference between the two groups (P <0.01) in a dose-dependent manner. (2) The apoptosis rates of endothelial cells treated with 30, 90 and 150 mg / L STBM for 24 h were (17.39 ± 1.11)%, (22.13 ± 1.02)% and (30.42 ± 1.98)%, respectively. 0.01) in a dose-dependent manner. Conclusion: STBM can inhibit the proliferation of HUVECs and induce the apoptosis of HUVECs, suggesting that it may be the cause of endothelial dysfunction in preeclampsia.