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为明确来自抗赤霉病的小麦品种望水白的2个主效抗扩展3B-QTL和6B-QTL的抗性遗传和互作模式,基于分子标记辅助选择方法,构建了回交分离群体,以病小穗数(NDS)和病轴长(LDR)为鉴定指标,采用单花滴注接种法对携带3B-QTL和6B-QTL的BC_3F_1、BC_3F_2、BC_3F_3世代以及抗感对照进行了抗赤霉病扩展的表型鉴定和评价。结果表明,3B-QTL和6B-QTL在烟农19和矮抗58不同的背景中,杂合基因型与纯合望水白基因型之间的NDS和LDR差异显著;携带3B-QTL和6B-QTL株系的抗扩展性与只含有单个3B-QTL的株系无显著差异,但显著高于只携带单个6B-QTL株系的抗性,抗感分离比经卡方检验符合4∶3∶9的分离比例,遵循2个独立孟德尔遗传因子控制的隐性遗传模式,且3B-QTL隐性上位于6B-QTL。研究表明,望水白的3B-QTL和6B-QTL抗扩展效应强且稳定,可以作为抗赤霉病基因资源在育种实践中充分利用。
To identify the inheritance and interaction patterns of resistance and inheritance of two main effect-resistant extended 3B-QTLs and 6B-QTLs from resistant wheat variety Wangshuibai, a backcross segregation population was constructed based on molecular marker- The index of disease spike number (NDS) and disease axis length (LDR) were evaluated by single flower inoculation method to test the resistance of BC_3F_1, BC_3F_2, BC_3F_3 generation and anti-susceptible control carrying 3B-QTL and 6B-QTL Phenotypic identification and evaluation of mycosis expansion. The results showed that the difference of NDS and LDR between 3B-QTL and 6B-QTL was significant in different backgrounds of Yannong 19 and Yingkang 58. The difference of NDS and LDR between 3B-QTL and 6B- QTL lines showed no significant difference in resistance to expansion but only a single 3B-QTL line, but were significantly higher than those lines carrying only a single 6B-QTL line. The anti-susceptibility segregation ratio was 4: 3: 9 segregation ratio followed the recessive inheritance mode controlled by two independent Mendelian genetic factors and the 3B-QTL was recessively located on the 6B-QTL. Studies have shown that Wangshuibai 3B-QTL and 6B-QTL anti-expansion effect is strong and stable, can be used as a scab resistance gene resources in breeding practice to take full advantage of.