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目的:研究大鼠脑出血后血肿周围组织水通道蛋白-4(AQP-4)的动态表达与血脑屏障通透性的相关性。方法:42只Sprague-Dawley大鼠随机分为假手术组、脑出血后6h、12h、1d、3d、7d、14d组,每组6只。用自体动脉血注入尾状核法建立大鼠脑出血模型,分别用免疫组织化学法和Evans蓝法检测大鼠脑出血后不同时间点血肿周围AQP-4和血脑屏障通透性的动态变化。结果:(1)血肿周围AQP-4在脑出血后12h表达开始增强,1d时明显增强,3d达高峰,7d略高于正常,14d基本恢复正常(P<0·05);(2)血脑屏障通透性最高出现在脑出血后12h~1d,3d开始恢复,7d明显降低,14d基本恢复正常(P<0·05);(3)脑出血后血肿周围AQP-4的动态表达与血脑屏障通透性存在相关性(χ2mh=10·41,P<0·05)。结论:脑出血后血肿周围AQP-4的动态变化规律提示AQP-4表达与脑损伤的发生和发展密切相关,是引起出血性脑损伤的重要因素之一。
Objective: To investigate the relationship between the dynamic expression of aquaporin-4 (AQP-4) and the permeability of the blood-brain barrier after intracerebral hemorrhage in rats. Methods: Forty-two Sprague-Dawley rats were randomly divided into sham operation group, 6h, 12h, 1d, 3d, 7d and 14d after cerebral hemorrhage. The model of intracerebral hemorrhage was established by autologous arterial blood injection into the caudate nucleus. The dynamic changes of AQP-4 and BBB permeability around the hematoma at different time points after ICH were detected by immunohistochemistry and Evans blue staining respectively . Results: (1) The expression of AQP-4 around the hematoma began to increase at 12h after ICH, and significantly increased at 1d, reaching the peak at 3d, slightly higher than normal at 7d and returned to normal at 14d (P <0.05). (2) The highest level of brain barrier permeability appeared at 12h ~ 1d after ICH, recovered at 3d, decreased at 7d and returned to normal at 14d (P <0.05). (3) The dynamic expression of AQP- Blood-brain barrier permeability was correlated (χ2mh = 10 · 41, P <0.05). Conclusion: The dynamic changes of AQP-4 around the hematoma after intracerebral hemorrhage suggest that the expression of AQP-4 is closely related to the occurrence and development of brain injury, which is one of the important factors that cause hemorrhagic brain injury.