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采用HPLC 法测定含有小抗原决定簇混合物(MDM)的各种皮肤试剂、简单皮试试剂(SSTR)的成份及稳定性。所用层析柱为μ-Bondapak C18反相柱,流动相为15%或20%甲醇-0.01M 磷酸钾缓冲液(pH7),于波长225nm 处检测。
The HPLC method was used to determine the composition and stability of various dermal agents and simple skin test agents (SSTRs) containing small antigenic determinants (MDMs). The column used was a μ-Bondapak C18 reverse phase column with a mobile phase of 15% or 20% methanol-0.01M potassium phosphate buffer (pH 7) at a wavelength of 225 nm.