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目的:研究枳实消痞汤对化疗大鼠胃肠组织乙酰胆碱酯酶(AChE)、一氧化氮合酶(NOS)的影响。方法:以环磷酰胺腹腔注射造成化疗性胃肠功能障碍大鼠模型,以香砂枳术丸作为对照,用不同剂量枳实消痞汤进行治疗后,检测各组大鼠胃窦和回肠组织AChE、NOS的含量。结果:模型组大鼠胃窦和回肠组织AChE的含量显著减少;枳实消痞汤大、中、小剂量组和香砂枳术丸组均使大鼠胃窦和回肠组织AChE的含量升高。模型组大鼠胃窦和回肠组织NOS的含量比空白对照组增高;枳实消痞汤大、中、小剂量组和香砂枳术丸组均比模型组NOS的含量降低。结论:化疗可使大鼠胃窦和回肠组织乙酰胆碱(ACh)释放减少,一氧化氮(NO)合成增多。枳实消痞汤使化疗大鼠胃窦和回肠组织ACh的释放增加,NO的合成减少,可能是其促进化疗大鼠胃肠蠕动的作用机理之一。
OBJECTIVE: To study the effects of Qishixiaoyu decoction on acetylcholinesterase (AChE) and nitric oxide synthase (NOS) in the gastrointestinal tissues of chemotherapy rats. METHODS: The rat model of chemotherapy-induced gastrointestinal dysfunction was induced by intraperitoneal injection of cyclophosphamide. Xiangsha Yuzhu Pill was used as a control. After treatment with different doses of Gushi Xiaoying Decoction, the gastric antrum and ileum of rats in each group were examined. AChE, NOS content. Results: The contents of AChE in the gastric antrum and ileum of the model group were significantly reduced. The contents of AChE in the gastric antrum and ileum of the rats were increased in the large, medium, and small doses of Ganshixiaotang decoction and Xiangsha Yushu Pills group. The content of NOS in gastric antrum and ileum of the model group was higher than that of the blank control group. The contents of NOS in the large, medium and small doses of Ganshixiaoyutang and Xiangshazhizhu were lower than those in the model group. CONCLUSION: Chemotherapy can reduce the release of acetylcholine (ACh) and increase the synthesis of nitric oxide (NO) in the gastric antrum and ileum of rats. The release of ACh in the gastric antrum and ileum of the chemotherapy rats increased with Zhishi Xiaotan Decoction, and the synthesis of NO was reduced, which may be one of the mechanisms that promote the gastrointestinal motility in rats with chemotherapy.