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A novel material MnO2 nanosheet has been used as the support matrix for the immobilization of horseradish peroxidase (HRP). HRP entrapped in MnO2 nanosheet film exhibits facile direct electron transfer with the electron transfer rate constant of 6.86 s-1. The HRP/MnO2 nanosheet film gives a re- versible redox couple with the apparent formal peak potential (E0′) of -0.315 V (vs. Ag/AgCl) in pH 6.5 phosphate buffer solution (PBS). The formal potential E0′ of HRP shifts linearly with pH with a slope of -53.75 mV·pH-1, denoting that an electron transfer accompanies single-proton transportation. The immobilized HRP shows an electrocatalytic activity to the reduction of H2O2. The response time of the biosensor for H2O2 is less than 3 s, and the detection limit is 0.21 μmol·L-1 based on signal/noise = 3.
A novel material MnO2 nanosheet has been used as the support matrix for immobilization of horseradish peroxidase (HRP). HRP entrapped in MnO2 nanosheet film exhibits facile direct electron transfer with the electron transfer rate constant of 6.86 s-1. The HRP / MnO2 nanosheet The formal potential E0 ’of HRP shifts linearly with pH (E0’) -0.315 V (vs. Ag / AgCl) in pH 6.5 phosphate buffer solution with a slope of -53.75 mV · pH-1, denoting that an electron transfer accompanies single-proton transportation. The immobilized HRP shows an electrocatalytic activity to the reduction of H2O2. The response time of the biosensor for H2O2 is less than 3 s, and the detection limit is 0.21 μmol·L-1 based on signal / noise = 3.