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为明确不同光质对烤烟叶片衰老的作用及对初烤烟叶品质的影响。以云烟87为供试材料,在大田条件下通过覆盖不同颜色滤膜以获得不同光质,研究白光(W)、红光(R)、黄光(Y)、蓝光(B)和紫光(P)5种不同光质对烤烟成熟衰老过程中内源激素和膜脂过氧化的影响。结果表明:与对照(白光)相比,红光可显著提高烟叶中细胞分裂素(CTK)和赤霉素(GA)的含量,有效降低脱落酸(ABA)含量,蓝光和紫光不利于CTK含量的增加却提高了烟叶中ABA的含量,蓝光和紫光处理下生长素(IAA)含量下降趋势早于红光和黄光处理;红光和黄光处理的烟叶中过氧化氢酶(CAT)活性升高,降低了烟叶衰老过程中的过氧化氢(H2O2)含量,使丙二醛(MDA)的含量保持相对较低的水平,从而延缓植株的衰老,蓝光和紫光处理使叶片CAT活性降低,H2O2含量和MDA含量显著高于对照及其他处理,加速了植株的衰老进程;红光处理提高了烟叶的总糖含量、施木克值和钾氯比,黄光处理提高了总氮和烟碱含量,红光和黄光处理降低了蛋白质含量与糖碱比。总体而言,红光处理提高了烟叶中IAA、CTK和GA含量以及CAT活性,减少H2O2、MDA和ABA含量,延缓膜脂过氧化的发生,从而延缓叶片衰老进程,使烟叶的质量得到改善。
In order to clarify the effect of different light qualities on leaf senescence and its effect on the quality of flue-cured tobacco leaves. Taking Yunyan87 as test material, different color filters were covered to obtain different light qualities under field conditions. The effects of white light (W), red light (R), yellow light (Y), blue light (B) and violet light Effects of Five Light Quality Treatments on Endogenous Hormones and Membrane Lipid Peroxidation in Flue - cured Tobacco During Maturation and Senescence. The results showed that compared with the control (white light), red light could significantly increase the content of cytokinin (CTK) and gibberellin (GA) in tobacco leaf and reduce the content of abscisic acid (ABA) But IAA content in blue and purple treatments decreased earlier than that in red and yellow treatments. CAT activity in red and yellow treated tobacco leaves increased significantly Increased the content of H2O2 in leaves and decreased the content of malondialdehyde (MDA) in senescence of tobacco leaves, thus delayed the senescence of plants. The activity of CAT in leaves was reduced by blue and violet treatment, H2O2 content and MDA content were significantly higher than the control and other treatments, accelerated the process of senescence of the plants; red light treatment increased the total sugar content of tobacco leaves, Shiqike value and potassium chloride ratio, yellow light treatment increased total nitrogen and nicotine Content, red and yellow treatments reduced the protein content and the ratio of sugar to base. In general, red light treatment increased the content of IAA, CTK and GA, and the activity of CAT in tobacco leaves, decreased the contents of H2O2, MDA and ABA, delayed the occurrence of membrane lipid peroxidation, delayed the leaf senescence and improved the quality of tobacco leaves.