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目的探讨3,3-二吲哚基甲烷(3,3-Diindolylmethane,DIM)对胃癌细胞SGC-7901增殖和侵袭的抑制作用。方法用MTT法检测不同浓度(0、10、20、40、80μmol/L)DIM对胃癌细胞SGC-7901生长抑制情况;Transwell小室检测40μmol/L的DIM对胃癌SGC-7901细胞侵袭力的影响,Western blot检测胃癌细胞中血小板源性生长因子-D(PDGF-D)和β-catenin蛋白表达的变化,ELISA法检测培养液上清中MMP-2、MMP-9的浓度。结果不同浓度的DIM对胃癌细胞SGC-7901均有抑制作用(P<0.05),随着药物浓度增加,抑制作用增强;但40μmol/L和80μmol/L差异无统计学意义(P>0.05)。40μmol/L的DIM作用24 h后胃癌细胞SGC-7901侵袭力下降75.2%(125.4±10.8 vs 31.1±4.5,P<0.05),PDGF-D和β-catenin的表达分别下降了55.8%和52.5%(P<0.05),培养液上清中的MMP-2、MMP-9的浓度明显下降(23.5±2.6 vs 13.2±1.9;30.4±2.4 vs 15.8±2.1,P<0.05)。结论 DIM对胃癌细胞的增殖和侵袭有抑制作用,其机制可能与PDGF-D、β-catenin、MMP-2、MMP-9的表达下降有关。
Objective To investigate the inhibitory effect of 3,3-diindolylmethane (DIM) on the proliferation and invasion of gastric cancer cell SGC-7901. Methods MTT assay was used to detect the growth inhibition of gastric cancer cells SGC-7901 at different concentrations (0, 10, 20, 40, 80μmol / L). Transwell chamber was used to detect the invasiveness of SGC- Western blot was used to detect the expression of platelet-derived growth factor-D (PDGF-D) and β-catenin in gastric cancer cells. The concentrations of MMP-2 and MMP-9 in the culture supernatant were detected by ELISA. Results Different concentrations of DIM had inhibitory effects on SGC-7901 cells (P <0.05). The inhibitory effect was enhanced with the increase of drug concentration. However, there was no significant difference between 40μmol / L and 80μmol / L groups (P> 0.05). The invasiveness of SGC-7901 cells decreased by 75.2% (125.4 ± 10.8 vs 31.1 ± 4.5, P <0.05) after 24 h treatment with 40 μmol / L DIM, while the expressions of PDGF-D and β-catenin decreased by 55.8% and 52.5% (P <0.05). The concentrations of MMP-2 and MMP-9 in the culture supernatant decreased significantly (23.5 ± 2.6 vs 13.2 ± 1.9; 30.4 ± 2.4 vs 15.8 ± 2.1, P <0.05). Conclusion DIM can inhibit the proliferation and invasion of gastric cancer cells, which may be related to the decreased expression of PDGF-D, β-catenin, MMP-2 and MMP-9.