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目的探讨芬戈莫德(fingolimod,FTY720)对试验性自身免疫性脑脊髓炎(EAE)小鼠脑组织中一氧化氮(NO)含量和诱导型一氧化氮合酶(i NOS)表达的影响。方法 48只雌性C57BL/6小鼠随机平均分为3组,EAE组运用MOG35-55构建EAE小鼠模型;CFA组由生理盐水代替MOG35-55构建模型;FTY720干预组在EAE基础上给予FTY720腹腔注射,CFA组、EAE组给予生理盐水腹腔注射。HE染色和LBF染色观察炎症情况和脱髓鞘情况,ELLISA检测小鼠脑组织中的NO含量,RT-PCR检测的诱导型一氧化氮合酶(i NOS)mRNA表达。结果 FTY720组EAE小鼠较EAE组临床症状减轻,炎症程度和脱髓鞘程度减轻(P<0.05)。FTY720组小鼠脑组织NO含量较EAE组降低(P<0.05),i NOS mRNA表达量降低(P<0.05)。结论 FTY720能抑制EAE小鼠脑组织中i NOS mRNA表达,从而减少NO含量。
Objective To investigate the effect of fingolimod (FTY720) on nitric oxide (NO) and inducible nitric oxide synthase (iNOS) expression in brain of experimental autoimmune encephalomyelitis (EAE) mice . Methods Forty-eight female C57BL / 6 mice were randomly divided into three groups: EAE group, MOG35-55 group, EAE group, normal saline group instead of MOG35-55 group, FTY720 group, FTY720 group Injections, CFA and EAE groups were given intraperitoneal injection of saline. The inflammation and demyelination were observed by HE staining and LBF staining. The content of NO in brain tissue was detected by ELLISA, and the expression of inducible nitric oxide synthase (iNOS) mRNA was detected by RT-PCR. Results Compared with EAE group, the clinical symptoms, the degree of inflammation and the degree of demyelination in FTY720 EAE mice were relieved (P <0.05). NO content in FTY720 group was lower than that in EAE group (P <0.05), and iNOS mRNA expression was decreased (P <0.05). Conclusion FTY720 can inhibit the expression of iNOS mRNA in the brain of EAE mice and thus reduce the content of NO.