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将具有抗香蕉枯萎病菌活性链霉菌通过固体发酵的优化,提高其菌丝生长以及孢子产量,并选择合适的吸附载体,为后期的香蕉枯萎病大田生物防治研究提供技术基础。采用单因子和4因子3水平正交试验法优化白刺链霉菌BWL15-4菌株及不吸水链霉菌BWL58菌株的固态法发酵培养基配方,通过单因子试验探讨了初始pH、接种量以及培养温度对2株菌生长情况的影响,并以孢子存活量多少确定了混合菌株的最适载体。BWL58优化后的固态发酵条件为:pH7.0左右,接种量2%,发酵温度28℃。培养基配方为:无机盐水占大米重量的150%,黄豆粉占大米重量的5%,麸皮占大米重量的20%,沙土占大米重量的30%。BWL15-4优化后的固体发酵条件为:初始pH6.3左右,接种量3%,发酵温度29.5℃。培养基配方为无机盐水占大米重量的130%,黄豆粉占大米重量的10%,麸皮占大米重量的20%,沙土占大米重量的20%。按照此优化条件进行固态发酵,白刺链霉菌BWL58菌株及不吸水链霉菌BWL15-4菌株孢子含量可分别达到1013cfu/g和1012cfu/g数量级以上。从面粉、高岭土、非耕作层土中通过筛选得到了混合菌的最佳填料为非耕作层。当非耕作层土与混合菌以2:1的比例混合时,在常温下保存3个月后,孢子数只降低一个数量级。
Optimization of the fermentation of Streptomyces fumigatus against Fusarium oxysporum f.sp., and its mycelial growth and spore yield, and the selection of suitable adsorption carrier, provide the technical basis for the biological control of banana wilt disease in the future. Single-factor and four-factor three-level orthogonal test was used to optimize the solid-state fermentation broth formula of Streptomyces tricolor var. Baracoides BWL15-4 and Streptomyces hygroscopicus BWL58. The initial pH, inoculum size and culture temperature On the growth of the two strains of the situation, and to determine the optimum amount of spore vector mixture of carriers. BWL58 optimized solid-state fermentation conditions: about pH7.0, inoculum 2%, fermentation temperature 28 ℃. The medium formula is as follows: inorganic salt water accounts for 150% of the weight of rice, soybean flour accounts for 5% of the weight of rice, wheat bran accounts for 20% of the weight of rice, and sandy soil accounts for 30% of the weight of rice. BWL15-4 optimized solid fermentation conditions: initial pH6.3 or so, inoculum size 3%, fermentation temperature 29.5 ℃. The formula for the medium is that inorganic salt water accounts for 130% of the weight of the rice, the soybean flour accounts for 10% of the weight of rice, the bran accounts for 20% of the weight of rice and the earth accounts for 20% of the weight of rice. Under these optimized conditions, the spore contents of Streptococcus sobrinus BWL58 and Streptomyces hygroscopicus BWL15-4 could reach 1013cfu / g and 1012cfu / g respectively. From the flour, kaolin, non-tillage layer of soil obtained by screening the best filler for non-tillage layer. When non-tillable soil and mixed bacteria were mixed in a 2: 1 ratio, the number of spores decreased by only one order of magnitude after 3 months of storage at room temperature.