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目的观察新型制剂回阳生肌脂质体凝胶促进慢性皮肤溃疡愈合的药效,探讨“回阳生肌”外用药治疗中医阴证疮疡的初步药理机制。方法SD大鼠80只随机分4组:空白对照组、模型组、传统回阳生肌膏治疗组和回阳生肌脂质体凝胶治疗组。采用“激素干预—皮肤缺损—细菌感染”复合因素叠加法制备慢性皮肤溃疡大鼠模型。实验观察大鼠一般生活行为,检测创面分泌物溶菌酶含量,血清肿瘤坏死因子-α(TNF-α)及血栓素B2(TXB2)含量及组织病理改变。结果模型组大鼠表现出中医临床“阴证”的基本证候特征。与模型组比较,传统回阳生肌膏及其脂质体凝胶治疗组创面分泌物溶菌酶含量显著升高(P<0.05或P<0.01);血清中TNF-α和TXB2的含量显著下降(P<0.05或P<0.01);创面肉芽组织增多,胶原合成旺盛,皮肤结构趋于完整。结论新型制剂回阳生肌脂质体凝胶在抗菌、消炎、促进胶原合成及创面愈合方面均表现出较好的疗效。
OBJECTIVE: To observe the efficacy of a new formulation of yangyangsheng liposome gel in promoting the healing of chronic skin ulcers, and to explore the preliminary pharmacological mechanism of “Yangyang Shengji ” external medicine in the treatment of traditional Chinese medicine syndromes and sores. Methods 80 SD rats were randomly divided into 4 groups: blank control group, model group, traditional Huiyang Shengjiu treatment group and Huiyang Shengji liposome gel treatment group. A rat model of chronic skin ulcers was prepared using the “hormone intervention—skin defect—bacterial infection” compound factor superposition method. Experiments were conducted to observe the general living behaviors of rats. The content of serum TNF-α, thromboxane B2 (TXB2) and histopathological changes were measured. Results The rats in the model group showed the basic syndrome characteristics of TCM clinical “negative syndrome ”. Compared with the model group, the content of lysozyme in the wound secretion of traditional Huiyang Shengji ointment and liposome gel treatment group was significantly increased (P<0.05 or P<0.01); the content of TNF-α and TXB2 in serum was significantly decreased. (P<0.05 or P<0.01); The granulation tissue increased, the collagen synthesis was vigorous, and the skin structure became complete. Conclusion The new formulation of Huiyang Shengji Liposome Gel showed good efficacy in antibacterial, anti-inflammatory, collagen synthesis and wound healing.