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应用兔抗纤维粘(连)蛋白(FN)抗体和兔抗3t,5,环腺苷酸(cAMP)特异性抗体,并用羊抗兔IgG荧光抗体,在荧光显微镜下观察丁酸钠对人胃腺癌SGC一7901细胞系的纤维粘(连)蛋白(FN)和cAMP水平的变化。实验结果表明丁酸钠有增强SGC一7901细胞内纤维粘(连)蛋白(FN)和c.~MP水平的作用;应用电镜细胞化学和扫描电镜的技术方法,观察到人胃腺癌SGC一7901细胞在丁酸钠作用下膜表而(Na+一K+)一ATP酶活性明显下降;微绒毛明显减退,膜表面较光滑。这可能说明丁酸钠通过抑制(Na~一K+)一ATP酶活性,提高了细咆内cAMP水平,调控细胞的增殖和分化。
Using rabbit anti-fibronectin (FN) antibody and rabbit anti-3t,5, cAMP specific antibody, and using goat anti-rabbit IgG fluorescent antibody to observe sodium butyrate against human gastric gland under fluorescence microscope Changes in fibronectin (FN) and cAMP levels in the cancer SGC-7901 cell line. The experimental results showed that sodium butyrate enhanced the level of fibronectin (FN) and c.~MP in SGC-7901 cells. The technique of electron microscopy and scanning electron microscopy was used to observe human gastric adenocarcinoma SGC-7901. The activity of (Na+-K+)-ATPase in the membrane under the sodium butyrate decreased significantly; the microvilli decreased significantly and the membrane surface was smooth. This may indicate that sodium butyrate, by inhibiting the activity of (Na~K+)-ATPase, increases the level of cAMP in fine oysters and regulates cell proliferation and differentiation.