对酶促反应色谱分离耦合过程进行了反应动力学参数及操作参数分析，并以蔗糖酶促水解为模型反应进行了正交实验研究。结果表明，该耦合过程对产物非竞争性抑制的消除效果要优于竞争性抑制。产率对底物浓度最为敏感，其次为洗脱速率和进料体积，而转化率的敏感性次序由大到小依次为洗脱速率、底物浓度和进料体积。 The reaction kinetic parameters and operating parameters were analyzed for the coupling reaction of the enzymatic reaction. The enzymatic hydrolysis of sucrose was used as model reaction to conduct orthogonal experiment. The results show that this coupling process is superior to competitive inhibition in eliminating the non-competitive inhibition of the product. The yield was the most sensitive to the substrate concentration, followed by the elution rate and the feed volume, while the order of sensitivity of the conversion order was the eluting rate, substrate concentration and feed volume in descending order.