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目的明确胎儿动脉导管、主动脉及肺动脉平滑肌细胞的增殖及凋亡特性以及相应的ERK1/2和JNK细胞信号传导通路是否存在差异,以及不同胎龄的平滑肌细胞在增殖凋亡及相应细胞信号传导通路是否有差异。方法取6例(18±2)周流产胎儿的主动脉、肺动脉和动脉导管进行细胞培养。通过原位免疫组织化学检测主动脉、肺动脉和动脉导管平滑肌细胞的PCNA、Bax和Bcl-2蛋白表达和通过ELIASA方法检测ERK1/2和JNK细胞信号传导通路的活化(磷酸化)状况。结果①在动脉导管,PCNA、Bcl-2、ERK表达16周胎龄最强,20周胎龄最弱,随胎龄增长而减弱,Bcl-2有统计学意义。不同胎龄主动脉、肺动脉各项指标无差异;②PCNA的表达肺动脉表达最强,主动脉次之,动脉导管最弱;③动脉导管平滑肌细胞在体外培养时比主动脉和肺动脉平滑肌细胞Bax/Bcl-2的比值高;④ERK1/2通路的OD值动脉导管表达最弱,主动脉表达最强。JNK通路的OD值动脉导管表达最强,主动脉表达最弱。结论胎儿动脉导管、主动脉及肺动脉平滑肌细胞在增殖凋亡和其相应的细胞传导通路有着明显的差异,动脉导管平滑肌细胞的生长状况可能随着胎龄的增长而减弱,与出生后动脉导管的闭合是否有关值得我们进一步探讨。
OBJECTIVE: To investigate the proliferation and apoptosis of fetal arterioles, aorta and pulmonary artery smooth muscle cells and the corresponding signal transduction pathways of ERK1 / 2 and JNK, as well as the proliferation and apoptosis of corresponding smooth muscle cells and corresponding cell signaling Pathways are different. Methods The aorta, pulmonary artery and ductus arteriosus of 6 fetuses (18 ± 2) weeks of abortion were cultured. The expressions of PCNA, Bax and Bcl-2 in aorta, pulmonary artery and arterial ductal smooth muscle cells were detected by in situ immunohistochemistry. The activation (phosphorylation) of ERK1 / 2 and JNK cell signal transduction pathway was detected by ELIASA. Results ① The expression of Bcl-2 in ductus arteriosus, PCNA, Bcl-2 and ERK was the strongest at 16 weeks, the weakest at 20 weeks and the weakened at gestational age. There was no difference in indexes of aorta and pulmonary artery at different gestational ages; ②PCNA expression was the strongest in pulmonary arteries, aorta was the second, arterial catheter was the weakest; ③ Arterial ductal smooth muscle cells were significantly higher than that of aorta and pulmonary artery smooth muscle cells Bax / Bcl -2 ratio; ④ ERK1 / 2 pathway OD value of the weakest duct, the strongest aortic expression. The OD value of JNK pathway was the highest in the ductus arteriosus and the weakest in the aorta. CONCLUSION: There is a significant difference between the proliferation and apoptosis of the smooth muscle cells in the ductus arteriosus, aorta and pulmonary artery, and their corresponding cell conduction pathways. The growth of the ductus arteriosus smooth muscle cells may be weakened with the increase of gestational age, Whether the closure is worthy of our further discussion.