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目的探讨丙泊酚对肺动脉平滑肌细胞跨膜钙离子移动的影响。方法SD大鼠麻醉后迅速取出心肺,分离肺动脉平滑肌细胞,进行体外培养。应用Fluo-3/AM进行荧光标记,采用激光共聚焦显微镜观察并测定有或无丙泊酚时,氯化钾(KCl)以及去氧肾上腺素(PE)对细胞内钙离子浓度的影响。结果KCl(60mmol/L)及PE(10-6mol/L)均能诱发体外培养的肺动脉平滑肌细胞内游离钙离子浓度的升高;加入50μmol/L丙泊酚后细胞荧光值由22.0±9.2降至16.4±8.0(P﹤0.01)。丙泊酚可显著抑制KCl及PE诱发的细胞内钙离子浓度的升高,荧光强度倍率分别由7.11±3.56降至3.43±0.79、1.35±0.14。结论丙泊酚对肺动脉平滑肌细胞膜上钙离子通道存在一定的抑制作用。
Objective To investigate the effect of propofol on transmembrane calcium mobilization in pulmonary artery smooth muscle cells. Methods SD rats were quickly removed after anesthesia, pulmonary artery smooth muscle cells were isolated and cultured in vitro. Fluorescence labeling was performed with Fluo-3 / AM. The effect of potassium chloride (KCl) and phenylephrine (PE) on intracellular calcium concentration was observed by laser confocal microscopy and the presence or absence of propofol was measured. Results Both KCl (60 mmol / L) and PE (10-6 mol / L) induced the increase of intracellular free calcium concentration in pulmonary artery smooth muscle cells cultured in vitro. The fluorescence intensity of cells increased from 22.0 ± 9.2 after adding 50μmol / L propofol To 16.4 ± 8.0 (P <0.01). Propofol could significantly inhibit the increase of intracellular calcium concentration induced by KCl and PE. The fluorescence intensity ratio decreased from 7.11 ± 3.56 to 3.43 ± 0.79 and 1.35 ± 0.14, respectively. Conclusion Propofol has some inhibitory effect on calcium channel in pulmonary artery smooth muscle cells.