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目的探讨2003年山东济南地区病毒性脑炎(VE)流行病因。方法通过随机PCR及肠道病毒特异性的RT-PCR扩增获得病毒分离株特异性核酸片段,测序并进行同源性分析和多序列比对,确定病毒种类及分型。结果从患者标本中分离到5株病毒,以随机PCR获得其中1株病毒的核酸片段,经BLAST分析发现其与肠道病毒同源性最高,然后分别测得5株病毒5′端非编码区及部分VP1区核酸序列,经序列比对分析,确定5株病毒均为小RNA病毒科肠道病毒属的柯萨奇B5,并且均与2002—2004年间浙江无菌性脑膜炎流行期间分离的柯萨奇B5分离株同源性最高(95%以上)。结论柯萨奇B5是2003年济南地区VE流行的重要病因之一。
Objective To explore the etiological factor of viral encephalitis (VE) in Shandong Jinan in 2003. Methods The specific nucleic acid fragments of virus isolates were obtained by random PCR and enterovirus specific RT-PCR amplification. Sequencing and homology analysis and multiple sequence alignment were performed to determine the virus types and types. Results Five strains of viruses were isolated from the patient samples. One of them was obtained by random PCR. The BLAST analysis showed that the two strains had the highest homology with the enterovirus, and then the 5 non-coding regions And part of the VP1 region nucleic acid sequences. Through sequence alignment analysis, all the 5 viruses were identified as Coxsackie B5 of the genus Parvovirus of the Picornaviridae and were isolated from the epidemic period of aseptic meningitis in Zhejiang during 2002-2004 Coxsackie B5 isolates with the highest homology (95% or more). Conclusion Coxsackie B5 is one of the important causes of VE in Jinan in 2003.