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目的比较液相萃取和固相萃取对毛发中海洛因毒品代谢物分析的影响。方法对海洛因吸食者毛发和空白添加标准品毛发经甲醇超声后的提取液分别进行液相萃取、固相萃取,然后进行衍生化和GC/MS-SIM检测。结果利用固相萃取法对添加6-单乙酰吗啡的毛发进行萃取和测试,6-单乙酰吗啡的回收率为32.0%,相对标准偏差(RSD)为2.4%;而液相萃取回收率为52.6%,相对标准偏差(RSD)为4.6%。结论固相萃取较之液液萃取,有更好的重复性,更少的杂质干扰和有机溶剂消耗等优势,但甲醇超声液需要挥干后才能进行固相萃取,而且6-单乙酰吗啡的水解率高。
Objective To compare the effects of liquid phase extraction and solid phase extraction on the analysis of heroin metabolites in hair. Methods The standard hair was added to the hair of the heroin addicts and the blank hair was subjected to liquid phase extraction and SPE followed by derivatization and GC / MS-SIM. Results The hair of 6-monoacetylmorphine was extracted and tested by solid-phase extraction. The recovery of 6-monoacetylmorphine was 32.0% and the relative standard deviation (RSD) was 2.4%. The recovery of liquid-phase extraction was 52.6 %, And the relative standard deviation (RSD) was 4.6%. Conclusion Solid-phase extraction has better repeatability, less impurity interference and organic solvent consumption than liquid-liquid extraction. However, methanol-based ultrasonication needs to be evaporated before solid-phase extraction can be carried out. Moreover, 6-monoacetylmorphine High hydrolysis rate.