论文部分内容阅读
利用美国大豆品种Clark(供体亲本)与主栽品种红丰11(轮回亲本)所构建的回交导入系,经过严格的芽期耐低温筛选鉴定,得到46个在芽期耐低温性状上明显超过轮回亲本的导入系个体。利用这套选择群体结合随机对照群体和基因型分析,通过基于遗传搭车原理的卡方分析和单向方差分析方法,检测到分布于大豆8个连锁群的12个与大豆芽期耐低温相关的QTL。其中卡方分析检测到9个供体片段的超导入位点,对芽期耐低温性状表现为正效应。方差分析检测到5个位点,也表现为正效应,且其中Satt237和SOYPRP1两个位点是两种方法共同检测到的,应视为与耐低温直接相关的QTL。本研究旨在创建大豆芽期耐低温分子育种的检测方法平台,为大豆芽期耐低温研究提供有用的分子标记。
The backcross inbred lines constructed by Clark (donor parent) and Hongfeng 11 (reincarnated parent) of the American soybean variety were identified through rigorous screening of low temperature resistant at bud stage, and 46 cultivars with obvious cold tolerance at bud stage were obtained More than the reincarnation of the introduction of the Department of the individual. Using this randomized control group and genotype analysis, we detected 12 low-temperature-resistant soybean buds distributed in 8 linkage groups of soybean through chi-square analysis and one-way analysis of variance based on genetic ride principle QTL. Among them, chi-square analysis detected the superconducting sites of 9 donor fragments and showed positive effect on low-temperature resistant traits at bud stage. Variance analysis detected five loci, also showed a positive effect, and Satt237 and SOYPRP1 two loci are detected jointly by the two methods, and should be regarded as low temperature directly related to QTL. The aim of this study was to create a platform for the detection of low temperature molecular breeding in soybean buds and to provide useful molecular markers for the study of low temperature resistance in soybean bud.