论文部分内容阅读
大黄性味苦寒,入胃经,易伤胃气,是脾阳虚证动物模型的常用造模因素,但未见其用于细胞水平的证候学研究,我们观察大黄酸对人胃粘膜上皮细胞系和大鼠原代胃粘膜上皮细胞生长的影响。 1 材料和方法 1.1 材料大黄酸由中国药品生物制品检定所提供(批号:0757-9703)。人胃粘膜上皮细胞系(GES-1)由北京市肿瘤防治研究所细胞遗传室提供。用MCDB151完全培养基培养,接种于塑料培养瓶中,内置无菌玻璃盖片,或接种干96孔板,1×10~3个/孔,0.1mL培养液/孔,置于37℃,50mL·L~(-1)CO_2细胞培养箱培养。完全培养基配制:取MCDB151培养基(Sigma)粉剂溶于三蒸水,加庆大霉素80kU·L~(-1),补加各种氨基酸成分,调pH至7.4。过滤除菌后,用前添加多种生长因子及生长促进剂,详见文献[3,4]。大鼠胃粘膜上皮细胞原代培养:雌性,Wistar大鼠,体质量95g～100g,由中国中医研究院实验动
Rhubarb is bitterly cold, ingested into the stomach, and easily injurious of the stomach. It is a commonly used modeling factor for animal models of spleen-yang deficiency, but it has not been used for cell-level syndrome research. We observed rhein on human gastric epithelium. Effects of Cell Line and Rat Primary Gastric Epithelial Cell Growth. 1 Materials and methods 1.1 Materials Rhein was provided by China National Institute for the Control of Pharmaceutical and Biological Products (batch number: 0757-9703). The human gastric epithelial cell line (GES-1) is provided by the Cell Genetics Office of the Beijing Institute of Cancer Research. Incubate with MCDB151 complete culture medium, inoculate in plastic culture flasks, install sterile glass coverslips, or inoculate dry 96-well plates, 1x10~3 cells/well, 0.1 mL of culture medium/well, placed at 37°C, 50mL · L-1 carbon dioxide incubator culture. Complete medium preparation: take MCDB151 medium (Sigma) powder and dissolve in triple-distilled water, add gentamycin 80kU·L -1, add various amino acid components, adjust pH to 7.4. After filtration and sterilization, a variety of growth factors and growth promoters were added before use, as detailed in the literature [3,4]. Primary culture of gastric epithelial cells in rats: female, Wistar rats, body weight 95g to 100g, tested by China Academy of Traditional Chinese Medicine