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目的:探索精子DNA甲基化水平异常与早期自然流产的关系。方法:随机选取98例符合纳入标准的配偶不明原因流产或胚停育男性为研究对象,另选取健康孕前体检男性46例作对照。行精液常规、精子形态分析,以改良精子染色质扩散法检测精子DNA碎片化指数。精子DNA甲基化水平检测采用甲基化定量试剂盒,运用比色法检测。结果:与对照组相比,早期不明原因流产组大晕环精子百分率[(45.50±26.27)%vs(36.49±23.06)%]显著降低(P=0.038),精子头部异常比例[(77.08±12.21)%vs(81.09±10.89)%]显著增高(P=0.049),精子DNA甲基化水平[(0.47±0.33)vs(0.36±0.26)ng/μl]显著降低(P=0.035);并且精子DNA甲基化水平与大晕环精子百分率呈正相关(r=0.546,P<0.01)。多因素回归分析显示,精子头部异常是早期自然流产/胚停育的独立危险因素(OR=1.032,P=0.049),而精子高甲基化水平是早期自然流产/胚停育的保护因素(OR=0.244,P=0.03)。结论:精子DNA甲基化水平异常可能是不明原因早期自然流产或胚停育的原因之一。
Objective: To explore the relationship between sperm DNA methylation abnormalities and early spontaneous abortion. Methods: Ninety-eight male males who died of unexplained abortion or embryo mating were enrolled in this study. Forty-six male males were selected as controls. Routine sperm routine, sperm morphology analysis, to improve the sperm chromatin diffusion method to detect sperm DNA fragmentation index. Sperm DNA methylation level using methylation quantitative test kit, the use of colorimetric detection. Results: Compared with the control group, the percentage of big halo ring sperm [(45.50 ± 26.27)% vs (36.49 ± 23.06)%] in early unexplained abortion group significantly decreased (P = 0.038), and the proportion of sperm head abnormality [(77.08 ± (P = 0.049), sperm DNA methylation level (0.47 ± 0.33) vs (0.36 ± 0.26) ng / μl] was significantly lower (P = 0.035); and The level of sperm DNA methylation was positively correlated with the percentage of sperm halo (r = 0.546, P <0.01). Multivariate regression analysis showed that sperm head abnormalities were an independent risk factor for early spontaneous abortion / embryo suspension (OR = 1.032, P = 0.049), while sperm methylation was the protective factor of early spontaneous abortion / embryo suspension = 0.244, P = 0.03). CONCLUSION: Abnormal sperm DNA methylation may be one of the reasons for unexplained early spontaneous abortion or embryo development.