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目的：研究加温联合六亚甲基二乙酰胺（ＨＭＢＡ）对人粘液表皮样癌ＭＥＣ－１细胞的作用。方法：采用ＭＴＴ法及软琼脂克隆形成法观察加温联合ＨＭＢＡ对ＭＥＣ－１细胞的作用；并用免疫组化法检测加温及联合ＨＭＢＡ对多药耐药（ＭＤＲ）表达蛋白（Ｐ－ｇｐ）的影响。结果：加温联合ＨＭＢＡ对ＭＥＣ－１细胞有协同抑制作用。免疫组化染色显示，对照组及单用药组细胞Ｐ－ｇｐ中度表达；加温组细胞Ｐ－ｇｐ不表达；联合组为低度表达。结论：加温联合ＨＭＢＡ对粘液表皮样癌的治疗可能有一定价值。
Objective: To investigate the effect of warming combined with hexamethylene dimethylamide (HMBA) on human mucoepidermoid carcinoma MEC-1 cells. Methods: The effects of warming combined with HMBA on MEC-1 cells were observed by MTT method and soft agar colony formation assay. The expression of MDR protein (P-gp) Impact. Results: Warming combined with HMBA had synergistic inhibitory effect on MEC-1 cells. Immunohistochemical staining showed that P-gp was moderately expressed in the control group and single-agent group; P-gp was not expressed in the warm-up group; and low expression in the combined group. Conclusion: Warming combined with HMBA may have some value in the treatment of mucoepidermoid carcinoma.