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目的:研究白藜芦醇干预兔实验性骨关节炎模型动物后关节软骨、滑膜细胞白细胞介素-1β合成的变化,探讨其治疗骨关节炎的效果和作用机制。方法:36只新西兰大白兔,随机抽取6只,作为正常对照组(A组),其余30只参考文献方法,制备兔OA模型,术后4周,经X线和病理改变证实造模成功后,将造模动物随机分为:模型组(B组)、阳性药物对照组(C组)(双醋瑞因1 mg·kg-1·d-1)、受试药物高剂量组(D1组)(白藜芦醇120 mg·kg-1·d-1)、中剂量组(D2组)(白藜芦醇60 mg·kg-1·d-1)、低剂量组(D3组)(白藜芦醇30 mg·kg-1·d-1),每组6只。并按设定剂量每日灌胃给药,连续给药6周后,处死动物,切取股骨内髁软骨标本,脱水石蜡包埋后切片,用免疫组化法检测软骨细胞中IL-1β水平。结果:受试药物组(D1组、D2组、D3组)软骨细胞中IL-1β阳性细胞分别为(21.8±3.8)%,(26.1±13.3)%,(30.4±4.9)%,明显低于模型组(B组)(49.1±5.5)%和阳性药物对照组(C组)(36.0±4.0)%,(P<0.01)。结论:高、中剂量的白藜芦醇能抑制模型组动物软骨细胞合成IL-1β,其能力优于阳性对照药物。
OBJECTIVE: To study the effect of resveratrol on the synthesis of articular cartilage and synoviocyte interleukin-1β in experimental osteoarthritis of rabbits and to explore its therapeutic effect and mechanism on osteoarthritis. Methods: Thirty-six New Zealand white rabbits were randomly divided into six groups: normal control group (A group) and the remaining 30 reference rabbits. Rabbit OA model was established. After 4 weeks, the model was confirmed by X-ray and pathological changes The model animals were randomly divided into three groups: model group (group B), positive control group (group C) (diacerein 1 mg · kg -1 · d -1), high dose group D1 ) (Resveratrol 120 mg · kg -1 · d -1), middle dose (group D2) (resveratrol 60 mg · kg -1 · d -1), low dose (group D3) Resveratrol 30 mg · kg -1 · d -1), 6 rats in each group. The rats were sacrificed 6 weeks after continuous administration. Animals were sacrificed, the cartilage of the femoral condyle was cut, embedded in dehydrated paraffin, sliced, and the level of IL-1β in chondrocytes was detected by immunohistochemistry. Results: The number of IL-1β-positive cells in test group was (21.8 ± 3.8)%, (26.1 ± 13.3)% and (30.4 ± 4.9)%, respectively, which were significantly lower than those in group D1, D2 and D3 The percentage of model group (B group) was (49.1 ± 5.5)% and that of positive control group (C group) was 36.0 ± 4.0% (P <0.01). CONCLUSION: High and medium doses of resveratrol can inhibit the synthesis of IL-1β by chondrocytes in model animals, which is superior to the positive control drugs.