论文部分内容阅读
为了探究不同供氮水平下氮素利用相关酶的贡献程度,采用单株砂培培养,严格控制氮素等营养供应,用15N饲喂追踪。研究了氮高效油菜品系742谷氨酰胺合成酶(GS)和谷氨酸合成酶(GOGAT)交替抑制情况下,油菜的子粒氮素转运和积累量、氮素损失的情况以及对氮素利用率的影响。结果表明,氮胁迫与正常供氮2种情况下,在抑制GOGAT活性时,氮素利用效率最低,子粒中氮素转运比例最低,氮素损失最大,在抑制GS时影响较小。缺氮时GOGAT对子粒氮素积累、作物产量形成及作物体内氮素再利用影响很大,GS影响较小。同时发现在生育后期,正常供氮与氮胁迫2种情况下子粒中来源于营养器官前期积累的氮素达到50%~70%。油菜营养器官生育前期积累的氮素主要用于氮素再利用,子粒中积累的氮素大部分来源于营养器官。
In order to explore the contribution of nitrogen-related enzymes under different levels of nitrogen supply, single sand culture was used to strictly control nitrogen and other nutrient supply, followed by 15N feeding. The nitrogen translocation and accumulation of rapeseed, nitrogen loss and the effects of nitrogen use efficiency on the reciprocal inhibition of glutamine synthetase (GS) and glutamate synthase (GOGAT) of nitrogen-efficient rapeseed line 742 were studied. Impact. The results showed that under the conditions of nitrogen stress and normal nitrogen supply, the nitrogen utilization efficiency was the lowest when the GOGAT activity was inhibited. The nitrogen transport rate was the lowest in the kernels and the nitrogen loss was the largest. Nitrogen deficiency GOGAT on grain nitrogen accumulation, crop yield formation and crop nitrogen reuse a great influence, GS less affected. At the same time, it was found that in the later stage of fertility, the amount of nitrogen accumulated in pre-vegetative organs reached 50% -70% in the two conditions of normal nitrogen supply and nitrogen stress. Nitrogen accumulated during the early growth stage of rape vegetative organs is mainly used for nitrogen reutilization, and most of the nitrogen accumulated in the grains comes from vegetative organs.