论文部分内容阅读
观察丹参及生脉液对阿霉素所致 Ｓ Ｄ 大鼠肾小球硬化实验性治疗作用。摘除大鼠左肾７ ｄ 后，尾静脉注射阿霉素（６ ｍ ｇ·ｋｇ － １） 。隔日腹腔注射丹参及生脉液，８ 周后处死大鼠。观察血红蛋白（ Ｈｂ） 、血尿素氮（ Ｂ Ｕ Ｎ） 、胆固醇（ Ｃｈ） 及２４ ｈ 尿蛋白含量； Ｅ Ｌ Ｉ Ｓ Ａ 法测定肾皮质Ⅳ型胶原（ Ⅳｃｏｌ ．） 及层粘连蛋白（ Ｌ Ｎ） ；用免疫组化法，计算机图象分析肾小球系膜区Ⅳｃｏｌ ．及 Ｌ Ｎ 含量。结果显示：模型丹参及模型生脉液组肾皮质、肾小球系膜区Ⅳｃｏｌ．及 Ｌ Ｎ 含量明显低于模型对照组（ Ｐ ＜ ０ ．０５） ，高于正常对照组（ Ｐ ＜ ０ ．０１） 。提示丹参及生脉液能减轻阿霉素所致肾小球硬化的程度。
Observe the experimental therapeutic effects of salviae miltiorrhizae and Shengmai liquid on adriamycin-induced SD rat glomerulosclerosis. After removal of the left kidney of rats for 7 days, doxorubicin (6 m g·kg -1) was injected into the tail vein. The intraperitoneal injection of salvia miltiorrhiza and shengmai liquid was performed every other day, and the rats were sacrificed 8 weeks later. Observation of hemoglobin (Hb), blood urea nitrogen (BUN), cholesterol (Ch) and 24-hour urinary protein content; E L I S A method for determination of renal cortex type IV collagen (IVcol.) and laminin (LN) Using immunohistochemistry, computer image analysis of mesangial area IVcol. And L N content. The results showed that the model Salviae miltiorrhiza and the model Shengmai Liquid group were the renal cortex and glomerular mesangial area IVcol. The content of LN was significantly lower than that of the model control group (P < 0.05) and higher than that of the normal control group (P <0. 01). It is suggested that salvia miltiorrhizae and Shengmai liquid can reduce the degree of glomerulosclerosis caused by adriamycin.