Microbes play important roles in the transport and transformation of selenium (Se) in the environment,thereby influencing plant resistance to Se and Se accumulation in plant.The objectives are to characterize the bacteria with high Se tolerance and reduction capacity and explore the significance of microbial origins on their Se tolerance,reduction rate and efficiency.Two bacterial strains were isolated from a naturally occurred Se-rich soil at tea orchard in south Anhui Province,China.The reduction kinetics of selenite was investigated and the reducing product was characterized using scanning electron microscopy and transmission electron microscopy-energy dispersive spectroscopy.The bacteria were identified as Lysinibacillus xylanilyticus and Lysinibacillus macrolides,respectively,using morphological,physiological and molecular methods.The results showed that the minimal inhibitory concentrations (MICs) of selenite for L.xylanilyticus and L.macrolides were 120 and 220 mmol/L,respectively,while MICs of selenate for L.xylanilyticus and L.macrolides were 800 and 700 mmol/L,respectively.Both strains aerobically reduced selenite with an initial concentration of 1.0 mmol/L to elemental Se nanoparticies (SeNPs) completely within 36 hr.Biogenic SeNPs were observed both inside and outside the cells suggesting either an intra-or extracellular reduction process.Our study implied that the microbes from Se-rich environments were more tolerant to Se and generally quicker and more efficient than those from Se-free habitats in the reduction of Se oxyanions.The bacterial strains with high Se reduction capacity and the biological synthesized SeNPs would have potential applications in agriculture,food,environment and medicine.