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为了探索花生属异源多倍体进化理论和种间杂交过程所涉及的遗传机制,以四倍体栽培种花生与二倍体野生种A.doigoi及其种间杂种F1和早期多倍体世代(S0~S3)为材料,采用cDNA-SCoT技术研究花生属人工异源多倍体进化早期基因表达变化规律。12条SCoT引物共扩增出108个cDNA片段,获得差异片段80个,占扩增总条带数的74.07%,对其中的35个差异片段进行克隆测序,有26个和GenBank数据库中已录入的基因具有较高的相似性,包括能量与代谢相关基因(8个)、未知功能蛋白基因(3个)、抗逆性相关基因(4个)、信号传导相关基因(2个)和反转录转座子相关基因(9个)。这说明花生属种间杂交人工异源多倍化早期世代发生着快速、剧烈的基因表达变化;从中获得的一些差异基因片段可用于花生属异源多倍化的分子机制研究。
In order to explore the genetic mechanism involved in heteropolyploid evolution and the interspecific hybridization of peanut genotypes, the tetraploid cultivars A.doigoi and its interspecific hybrid F1 and early polyploid generation (S0 ~ S3) were used as materials to study the changes of early gene expression in artificial heterologous polyploid evolution of peanut by cDNA-SCoT. A total of 108 cDNA fragments were amplified with 12 SCoT primers and 80 differential fragments were obtained, accounting for 74.07% of the total number of amplified bands. 35 of them were cloned and sequenced, of which 26 were cloned and sequenced in GenBank database (8 genes), 3 unknown functional protein genes (4 genes), 4 genes related to stress resistance (2 genes) and 2 Transposon related genes (9). This indicates that rapid and dramatic changes of gene expression occur in the early generation of artificial allogamy of peanut hybrids. Some differential gene fragments can be used to study the molecular mechanism of allopolyploidization of peanut.