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为明确安徽水稻主栽区稻曲病菌群体遗传结构、致病力及相互对应关系,从安徽28个水稻种植县(市)采集并分离稻曲病菌92株,利用REP-PCR(repetitive extragenic palindromic sequence PCR)进行菌株遗传多样性分析并采用人工注射接种测定致病力。结果表明,安徽稻区稻曲病菌群体遗传多样性丰富,在0.76相似性水平上可分为7个族,分属于不同族的24个菌株致病力差异显著,但与地域来源与该试验采用相关方法进行的归类或分簇无对应关系,菌株致病力与水稻品种表现出明显的特异性。
In order to clarify the genetic structure, virulence and mutual relationship of the rice false smut populations in the main rice planting area of Anhui province, 92 strains of Y. traspora were collected and isolated from 28 rice planting counties in Anhui province. PCR) was used to analyze the genetic diversity of the strains and the pathogenicity was determined by artificial inoculation. The results showed that there were abundant genetic diversity in rice germplasm in Anhui Province, which could be divided into 7 groups at the similarity level of 0.76. The pathogenicity of 24 strains belonging to different groups was significantly different, but with the geographical origin and the test There is no corresponding relationship between taxonomic classification and clustering. The virulence of the strains showed obvious specificity with rice varieties.