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miRNAs作为一种基因表达调控子在植物应答胁迫的过程中起着重要的作用,当花生受到盐胁迫时,也会有相应的miRNAs参与基因表达调控应答胁迫。本研究对200份花生品种进行萌发期耐盐性鉴定,获得了高耐盐花生品种3份,中耐盐花生品种5份,盐高敏感品种4份。并对不同耐盐性花生品种在盐胁迫处理条件下进行了抗氧化酶活性(SOD,POD,CAT)和MDA含量的测定。结果表明,耐盐性花生品种清除活性氧的能力大于盐敏感型。盐胁迫条件下,耐盐性花生植株MDA含量较少,受到的伤害相对较小,而盐敏感植株的受伤害程度最大,也证明了耐盐性花生品种在受到盐胁迫伤害时植物体内存在更强大的保护作用。通过小RNA测序及对靶基因序列进行功能分析和同源序列功能检索,获得了8条花生耐盐相关保守miRNAs序列,miR159-1,miR159-2,miR159-3,miR164-2,miR167-3,miR319-1,miR319-2,miR2111-1。荧光定量PCR测定结果表明,这些花生保守miRNAs受盐胁迫诱导,并调控其靶基因的应答反应。
As a regulator of gene expression, miRNAs play an important role in plant response to stress. When peanuts are subjected to salt stress, corresponding miRNAs are also involved in stress response to gene expression and regulation. In this study, the identification of salt tolerance of 200 peanut varieties during the germination phase showed that 3 varieties of high salt tolerant peanut varieties, 5 medium salt tolerant peanut varieties and 4 high salt sensitive varieties were obtained. The antioxidant enzyme activities (SOD, POD, CAT) and MDA contents of peanut cultivars with different salt tolerance were measured under salt stress. The results showed that the ability of salt-tolerant peanut species to scavenge reactive oxygen species was greater than that of salt-sensitive species. Under salt stress, the content of MDA in salt-tolerant peanut plants was less, the damage was relatively less, and the salt-sensitive plants harmed to the greatest extent. It also proved that salt-tolerant peanut varieties had more damage to plants under salt stress Strong protection. Through small RNA sequencing and functional analysis of the target gene sequences and functional search of the homologous sequences, eight conserved miRNAs sequences related to salt tolerance were obtained, including miR159-1, miR159-2, miR159-3, miR164-2, miR167-3 , miR319-1, miR319-2, miR2111-1. Fluorescent quantitative PCR results showed that these peanut-conserved miRNAs were induced by salt stress and regulated the response of their target genes.