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目的分析FTO基因SNP rs9939609多态性与成年女性肥胖及其相关代谢指标的关系。方法随机选取-80℃保存的“2002年中国居民营养与健康状况调查”中1 250名28～60岁汉族成年女性的冻存静脉血,进行全基因组DNA抽提。采用Taqman-MGB探针技术进行FTO SNP rs9939609分型,其中1 245名成功完成分型。利用“2002年中国居民营养与健康状况调查”资料,分析不同基因型与汉族成年女性肥胖等指标之间的关系。结果 FTO SNP rs9939609 A等位基因表达频率为13.6%,AA、AT、TT基因型的表达频率分别为1.77%,23.69%和74.54%,各基因型的频数分布符合Hardy-Weinberg平衡(P=0.80);与TT基因型携带者相比,携带AA/AT基因型的汉族成年女性发生肥胖的危险性显著升高(OR=1.63,95%CI:1.13～2.44),FTO导致肥胖的人群归因危险度百分比(PAR%)为12.1%;携带AA/AT基因型与TT基因型的超重率、身高、体重、体质指数(BMI)、空腹血糖(FBG)、三酰甘油(TG)、总胆固醇(TC)、高密度脂蛋白胆固醇(HDL-C)、低密度脂蛋白胆固醇(LDL-C)、舒张压(SBP)、收缩压(DBP)等指标差异均无统计学意义。结论 FTO基因SNP rs9939609多态性与中国汉族成年女性肥胖存在相关性,携带AA/AT基因型的汉族成年女性发生肥胖的危险性显著高于TT基因型携带者,但未发现对其他代谢指标的影响。
Objective To analyze the relationship between FTO gene SNP rs9939609 polymorphism and obesity and its related metabolic parameters in adult women. Methods A total of 1 250 frozen female venous blood from 28 to 60-year-old Han women aged from 80 to 80 years were collected at -80 ℃ for whole genome DNA extraction from 2002-2002 Survey on Nutrition and Health Status of Chinese People. FTO SNP rs9939609 typing was performed using the Taqman-MGB probe technique, of which 1 245 were successfully typed. Using the “2002 China Nutrition and Health Survey” information, analysis of different genotypes and adult Han women obesity and other indicators. Results The frequency of allele A of FTO SNP rs9939609 was 13.6%, and the frequencies of AA, AT and TT genotypes were 1.77%, 23.69% and 74.54%, respectively. The frequency distribution of each genotype was in accordance with Hardy-Weinberg equilibrium (P = 0.80 ). Compared with TT genotype carriers, adult Han women with AA / AT genotype had a significantly increased risk of obesity (OR = 1.63, 95% CI: 1.13-2.44) Risk percentage (PAR%) was 12.1%. The prevalence of overweight, height, weight, body mass index (BMI), fasting blood glucose (FBG), triglyceride (TG), total cholesterol TC, HDL-C, LDL-C, SBP and DBP did not show statistical significance. Conclusion There is a correlation between SNP rs9939609 polymorphism in FTO gene and obesity in adult Han Chinese women. Han adults with AA / AT genotype have a significantly higher risk of developing obesity than TT genotype carriers, but no significant differences were observed in other metabolic markers influences.