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目的:研究N-乙酰半胱氨酸(NAC)雾化吸入对于博莱霉素(BLM)导致的大鼠肺纤维化的影响作用。方法:取清洁SD大鼠100只,平均分为10小组,1小组为对照组,其余每3小组为一组,分别为模型组,防治组,治疗组,通过在大鼠气管中注入BLM的方法来制造大鼠肺纤维化模型,防治组在造模前的7天以及造模后的7天内,治疗组在造模后的7天内,分别给予NAC雾化吸入治疗。在成功造模后的第7天、14天、28天,每组处死1小组大鼠,取出病理组织,通过免疫组化方法来观察肺组织纤维化的程度。研究NAC对大鼠肺纤维化的影响。结果:与模型组对比,防治组和治疗组在纤维化程度上明显较轻(P<0.05)。结论:吸入NAC对于博莱霉素导致的大鼠肺纤维化有明显的抑制作用,而且防治组疗效明显好于治疗组。既提前7天用药,效果更明显,大鼠获益更多。
AIM: To investigate the effect of inhaled N-acetylcysteine (NAC) on pulmonary fibrosis induced by bleomycin (BLM) in rats. Methods: One hundred SD rats were divided into 10 groups randomly. One group was control group, and the other three groups were model group, control group and treatment group. BLM was injected into rat trachea Methods The rat model of pulmonary fibrosis was established. The control group was given NAC inhalation treatment within 7 days after model establishment and within 7 days after modeling, respectively. On the 7th day, 14th day and 28th day after successful modeling, 1 group of rats were sacrificed and the pathological tissues were removed. The degree of lung fibrosis was observed by immunohistochemistry. To study the effect of NAC on pulmonary fibrosis in rats. Results: Compared with the model group, the degree of fibrosis in the control group and the treatment group was significantly lower (P <0.05). Conclusion: Inhalation of NAC significantly inhibited bleomycin-induced pulmonary fibrosis in rats, and the efficacy of the control group was significantly better than that of the treatment group. Both 7 days ahead of medication, the effect is more obvious, rats benefit more.