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利用流式细胞仪(FCM)技术检测了杂色鲍血细胞在体内和体外对不同含量荧光微球悬液的吞噬率.其结果表明体内注射法测定吞噬率的方法不可行.在体外吞噬中,当荧光微球含量为血细胞含量的68.2倍时吞噬率达98.43%±1.27%,随着荧光微球含量的降低血细胞吞噬率也随着降低.当荧光微球含量为血细胞含量的6.82倍时吞噬率为63.45%±6.05%,以这一含量进行血细胞吞噬率的测定比较恰当.利用这一含量测定了正常鲍与经过低盐(S=18)海水胁迫24 h后鲍的血细胞吞噬率的变化,正常鲍的吞噬率为68.71%±10.05%,经低盐海水胁迫后鲍的吞噬率为49.49%±10.18%,吞噬率出现了显著的下降(p<0.05).实验结果表明利用流式细胞仪技术相比传统的显微镜镜检记数法检测杂色鲍血细胞吞噬率更为方便可靠.
Flow cytometry (FCM) was used to detect the phagocytosis rates of Haliotis diversicolor haemocytes in vitro and in vivo, and the results showed that in vivo phagocytosis assay was not feasible.In vitro phagocytosis, The phagocytosis rate reached 98.43% ± 1.27% when the content of fluorescent microspheres was 68.2 times of the blood cell content, and the phagocytic rate of hemocytes decreased with the decrease of the content of fluorescent microspheres.When the content of fluorescent microspheres was 6.82 times of the blood cell content, (63.45% ± 6.05%), and it was appropriate to measure the rate of phagocytosis of blood cells with this content.The change of phagocytosis rate of normal hamster and abalone after 24 h of low salt (S = 18) seawater stress , The phagocytic rate of normal abalone was 68.71% ± 10.05%, the phagocytic rate of abalone was 49.49% ± 10.18% after low salt seawater stress, and the phagocytic rate was significantly decreased (p <0.05) .Experimental results showed that the flow cytometry Instrument technology compared to the traditional microscope microscopy count method to detect variegated hemocyte phagocytosis rate more convenient and reliable.