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目的:探讨S-亚硝基谷胱甘肽(GSNO)对腹部创伤大鼠肠黏膜屏障的保护作用。方法:建立腹部创伤大鼠模型,将24只大鼠随机分为对照组(腹部创伤组)和GSNO组(腹部创伤模型+GSNO干预,50μg/kg),每组12只。分别在第1、6和24 h检测肠黏膜紧密连接蛋白(Occludin、Zo-1、Claudin1/2)的表达水平;FITC-葡聚糖(FD4)对大鼠体内肠黏膜屏障的通透性;肠壁中胶质纤维酸性蛋白(GFAP)阳性细胞的数量。结果:GSNO作用1 h和6 h,血浆中FD4水平显著低于对照组(P<0.05);GSNO处理6 h、24 h,肠黏膜屏障中紧密连接蛋白Claudin、Occludin、Zo-1表达水平均显著高于对照组(P<0.01);GSNO作用6 h和24 h时,GFAP阳性细胞数均显著低于对照组(P<0.01)。结论:使用GSNO干预,可改善腹部创伤大鼠的肠黏膜屏障通透性增加和肠屏障功能损害。
Objective: To investigate the protective effect of S-nitrosoglutathione (GSNO) on intestinal mucosal barrier in traumatic rats. Methods: Twenty-four rats were randomly divided into control group (abdominal trauma group) and GSNO group (abdominal injury model + GSNO intervention, 50μg / kg), 12 rats in each group. The expressions of Occludin, Zo-1 and Claudin1 / 2 were detected at 1, 6 and 24 h, respectively. The permeability of FITC-dextran (FD4) to the intestinal mucosal barrier in rats was measured. The number of glial fibrillary acidic protein (GFAP) positive cells in the intestinal wall. Results: The levels of Claudin, Occludin and Zo-1 in the intestinal mucosal barrier of GSNO treatment for 6 h and 24 h were significantly lower than those of the control group (P <0.05) (P <0.01). At the 6th and 24th hour after GSNO treatment, the number of GFAP positive cells was significantly lower than that of the control group (P <0.01). Conclusion: GSNO intervention can improve the intestinal mucosal barrier permeability and intestinal barrier dysfunction in traumatized rats.