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ＲＦＬＰ是一种新型的遗传标记。在一个经过有性繁殖过程产生的分离群体中，只要利用有效的克隆探针进行检测，就能获得丰富的ＲＦＬＰ标记，再结合常规的遗传分析和专门的计算机软件处理，就可以构建一套在精密度上都比其它遗传连锁图更为先进的ＲＦＬＰ连锁图。利用ＲＦＬＰ连锁图及其检测技术可以在植物基因组中对一些控制重要农艺性状的主基因以及复杂的数量性状基因的位点进行准确的标记定位，从而能有效地进行重要农艺性状的遗传分析和作物改良，野生种质资源的利用以及种间杂交等研究，大大地提高植物育种的效率。利用人工酵母染色体和染色体逐段检测技术结合高密度的ＲＦＬＰ连锁图，可以将一些基因产物未知的目标基因克隆出来，以提高转基国植物创造的实用性。
RFLP is a new type of genetic marker. In a segregating population that has undergone a sexual reproduction process, abundant RFLP markers can be obtained by using efficient cloning probes for detection, combined with routine genetic analysis and specialized computer software processing, Precision than other genetic linkage map more advanced RFLP chain map. Using the RFLP linkage map and its detection technology, we can accurately mark and locate the major genes controlling important agronomic traits and the complex quantitative trait loci in the plant genome, so that the genetic analysis of important agronomic traits and crops Improvement, the utilization of wild germplasm resources and interspecific hybridization and other research, greatly enhance the efficiency of plant breeding. Using artificial yeast chromosomes and chromosomal piecewise detection techniques combined with high-density RFLP linkage maps, some target genes with unknown gene products can be cloned to improve the practicality of the creation of transgenic plants.