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本研究采用ABC免疫酶标和间接免疫荧光技术,观察了从未受刺激大鼠脾分离的富含淋巴树状突细胞组分内细胞的Ia抗原表达.结果表明,染色标本中主要有3类细胞:1、胞体大,表面伸出树枝状突起,ABC法染色时,呈棕褐色,免疫荧光染色时,发强淡绿色荧光.2、胞体较大,呈圆形或卵圆形,无明显突起,ABC法染色时,不着色或呈淡棕黄色,免疫荧光染色时,不发荧光或发淡黄绿色荧光。3.淋巴细胞,胞体大小不一,呈球形,染色结果与2类细胞相似.文章结合SEM观察和S-100蛋白染色结果论证了这三类细胞依次为淋巴树状突细胞、巨噬细胞和淋巴细胞。结果证实淋巴树状突细胞表面持续表达大量Ia抗原。
In this study, ABC immunoenzyme labeling and indirect immunofluorescence techniques were used to observe the expression of Ia antigen in lymphocyte-rich lymphocytes from splenocytes that had never been stimulated.Results showed that there were 3 types Cells: 1, large cell body, the surface protruding dendrites, ABC staining, was brown, immunofluorescence staining, strong light green fluorescence .2, cell body larger, round or oval, no significant Protrusions, ABC staining, no coloring or pale brown, immunofluorescence staining, no fluorescence or yellowish green fluorescence. 3. Lymphocytes with different size and spherical shape showed similar staining results to cell type 2. The combination of SEM and S-100 protein staining demonstrated that the three types of cells were lymphatic dendritic cells, macrophages and Lymphocytes. The results confirmed that the surface of lymphoid dendritic cells continue to express large amounts of Ia antigen.