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哺乳动物因为缺乏Δ-12和ω-3脂肪酸脱氢酶,不能自身合成必需的多不饱和脂肪酸.目前,通过转基因技术在哺乳动物体内表达ω-3脂肪酸脱氢酶,能将长链的n-6多不饱和脂肪酸转化成n-3多不饱和脂肪酸,造成体内长链的n-6多不饱和脂肪酸含量显著减低.本研究通过自我剪切2A肽介导Δ-12和ω-3脂肪酸脱氢酶(FAT-2和FAT-1)以及人过氧化氢酶(human catalase,hCAT)在小鼠的肌肉同时表达.结果表明,转基因小鼠肌肉中长链n-3多不饱和脂肪酸含量提高2.6倍,长链n-6多不饱和脂肪酸含量没有显著变化,而n-6/n-3比例显著降低(P<0.01).同时蛋白质印迹检测到人过氧化氢酶hCAT在小鼠的肌肉组织中表达,且过氧化氢酶活性比野生型小鼠显著提高(P<0.01).
Mammals are unable to synthesize the requisite polyunsaturated fatty acids themselves due to the lack of delta-12 and omega-3 fatty acid dehydrogenases Presently, expression of omega-3 fatty acid dehydrogenases in mammals by transgenic techniques allows long chain n -6 polyunsaturated fatty acids into n-3 polyunsaturated fatty acids, resulting in the body long chain n-6 polyunsaturated fatty acid content significantly reduced in this study by self-shearing 2A peptide mediated delta-12 and omega-3 fatty acids Dehydrogenase (FAT-2 and FAT-1) and human catalase (hCAT) were simultaneously expressed in muscle of mice.The results showed that the long-chain n-3 polyunsaturated fatty acid , While the ratio of n-6 / n-3 was significantly decreased (P <0.01) .While the protein expression of human catalase hCAT in mouse Muscle tissue, and catalase activity was significantly higher than wild-type mice (P <0.01).