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通过在除虫菊酯6种单体的五元环酮基上引入羧基的方法制备半抗原,再用碳二亚胺法偶联到牛血清白蛋白(BSA)上制得完全抗原。经紫外光谱测定,6种完全抗原的分子偶联比为6∶1(除虫菊酯Ⅰ:BSA)、13∶1(除虫菊酯II:BSA)、9∶1(瓜叶菊酯Ⅰ:BSA)、11∶1(瓜叶菊酯II:BSA)、7∶1(茉酮菊酯Ⅰ:BSA)和9∶1(茉酮菊酯II:BSA)。以完全抗原免疫BALB/c小鼠制抗血清,采用间接酶联检测抗体效价均达到105以上,再用饱和硫酸铵沉淀得到除虫菊酯的多克隆抗体。该结果为除虫菊酯的酶联抗体检测及免疫分析技术奠定了基础。
Hapten was prepared by introducing carboxyl groups into the five-membered ring ketones of 6 kinds of pyrethrins and then coupled to bovine serum albumin (BSA) by carbodiimide method to obtain the complete antigen. The molecular coupling ratios of 6 complete antigens were 6:1 (pyrethrin Ⅰ: BSA), 13:1 (pyrethrin II: BSA), 9:1 (cinense I: BSA), 11 : 1 (echinacea II: BSA), 7: 1 (imidacloprid I: BSA) and 9: 1 (imidacloprid II: BSA). BALB / c mouse antiserum was immunized with complete antigen, and the antibody titers were all above 105 by indirect enzyme-linked immunosorbent assay (ELISA). The polyclonal antibodies against pyrethrin were precipitated by saturated ammonium sulfate. The results laid the foundation for the detection of pyrethroid enzyme-linked antibodies and immunoassay techniques.