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目的 建立大鼠急性呼吸窘迫综合征动物模型 (ARDS)。方法 选取KM小鼠SD大鼠 (雌雄不限 ) ,分为正常对照组及百草枯组 ,小鼠按每公斤体重 10 0mg ,大鼠按每公斤体重 12 0mg一次灌胃给药。再分 2 4h、4 8h、72h组 ,小鼠每组 10只股动脉采血用于血气分析 ,取肺测定肺系数 ,全肺拍照进行对照 ,大鼠每组 10只取肺石腊包埋 ,病理切片。结果 ①整体外观变化 随着给药时间延长 ,呼吸变得越来越困难、消瘦、咳嗽 ,2 4h后鼻腔有淡红色分泌物 ,食欲渐进性下降。②肺外观观察炎症渐进性发展 ,从正常粉红色发展到最后呈肝样变。③血气变化 随染毒时间延长 ,血液pH值逐渐降低 ,PaCO2 逐渐升高 ,PaO2 逐渐下降 ,肺系数逐渐增大 ,组间数据进行t检验p <0 0 1,差异有显著性。④病理切片观察肺部呈典型炎症病理变化过程 ,最终肺泡内形成典型透明膜。结论 百草枯染毒成功制造鼠ARDS模型。
Objective To establish a rat acute respiratory distress syndrome animal model (ARDS). Methods KM male SD rats were randomly divided into normal control group and paraquat group. The mice were given 100 mg body weight per kg body weight and the rats were intragastrically administered with 120 mg body weight per kg body weight. Divided into 2 4h, 4 8h, 72h group, the mice in each group of 10 femoral artery blood for blood gas analysis, take lung measurement of lung coefficient, whole lung photographs for comparison, 10 rats in each group take the paraffin embedded, Pathological sections. Results ① The overall appearance of the changes with the delivery time, breathing becomes more and more difficult, weight loss, cough, nasal 24 hours after the light red secretions, appetite decreased. ② lung appearance observation of progressive development of inflammation, from the normal development of pink to the final liver-like change. ③ blood gas changes with the extension of exposure time, the blood pH value decreased, PaCO2 gradually increased, PaO2 gradually decreased, pulmonary coefficient gradually increased, the data between groups t test p <0 01, the difference was significant. Pathological observation of the lungs showed a typical inflammatory pathological changes in the process, the formation of the typical alveolar transparent membrane. Conclusion Paraquat-induced ARDS model was successfully established.