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目的研究 DNA 修复基因 XRCC3 Thr 241 Met 遗传变异与喉癌和下咽癌风险的关系。方法采用聚合酶链反应-限制性片段长度多态分析方法对175例喉癌及下咽癌患者和525名无肿瘤对照进行基因分型,采用多因素 Logistic 回归模型计算各基因型携带者喉癌和下咽癌的发病风险,以及与吸烟交互对喉癌下咽癌发病风险的影响。结果 XRCC3 241 Met 等位基因增加了喉癌、下咽癌发病风险,与 XRCC3 241 Thr/Thr 基因型携带者相比,至少携带一个241 Met 等位基因的个体罹患喉癌、下咽癌的比值比(odds ratio,OR)为2.26,95%可信区间(confidence interval,CI)为1.33～3.82。分别分析 XRCC3多态与喉癌及下咽癌发病风险关系发现,XRCC3 241 Met 等位基因均增加喉癌与下咽癌的发生风险,与 XRCC3 241 Thr/Thr 基因型携带者相比,至少携带一个 XRCC3 241 Met 等位基因的个体发生喉癌和下咽癌风险的 OR 值(95%CI)分别为2.27(1.26～4.09)和2.99(1.27～7.04)。基因吸烟交互作用分析结果显示,重度吸烟和 XRCC3 Thr 241 Met 多态存在相乘交互作用,显著增加喉癌、下咽癌发病风险。至少携带一个 XRCC3 241 Met 等位基因的重度吸烟个体发生喉癌、下咽癌的 OR 值(95%CI)为19.09(7.38～49.40),大于至少携带一个 XRCC3 241 Met 等位基因的不吸烟个体(OR,0.91;95%CI,0.20～4.21)及重度吸烟但携带 XRCC3 241 Thr/Thr 基因型个体(OR,4.13;95%CI,2.38～7.17)的 OR 乘积。结论 XRCC3 Thr 241 Met 单核苷酸多态是喉癌、下咽癌的遗传易感因素。
Objective To study the relationship between the genetic variation of DNA repair gene XRCC3 Thr 241 Met and the risk of laryngeal cancer and hypopharyngeal cancer. Methods Polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) was used to genotype 175 patients with laryngeal and hypopharyngeal carcinoma and 525 tumor-free controls. Multivariate Logistic regression model was used to calculate the laryngeal cancer And the incidence of hypopharyngeal cancer risk, as well as interaction with smoking on the incidence of hypopharyngeal cancer risk. Results The allele XRCC3 241 Met increased the risk of laryngeal cancer and hypopharyngeal cancer. Compared with carriers of the XRCC3 241 Thr / Thr genotype, individuals with at least one 241 Met allele had laryngeal and hypopharyngeal cancer The odds ratio (OR) was 2.26, 95% confidence intervals (CI) ranged from 1.33 to 3.82. The relationship between the polymorphism of XRCC3 and the risk of developing laryngeal carcinoma and hypopharyngeal carcinoma were analyzed. The results showed that both the XRCC3 241 Met allele and the XRCC3 241 Thr / Thr genotype carriers at least carry The odds ratio (95% CI) for the risk of laryngeal and hypopharyngeal cancer in individuals with a single XRCC3 241 Met allele was 2.27 (1.26 to 4.09) and 2.99 (1.27 to 7.04), respectively. Gene smoking interaction analysis showed that heavy smoking and XRCC3 Thr 241 Met polymorphism there is a multiplication interaction, significantly increased the risk of laryngeal cancer, hypopharyngeal cancer. The odds ratio (95% CI) of laryngeal cancer and hypopharyngeal cancer was 19.09 (7.38 ~ 49.40) in the smokers who carried at least one Met allele of XRCC3 241 Met allele, which was higher than that in non-smokers who carried at least one allele of XRCC3 241 Met (OR, 0.91; 95% CI, 0.20-4.21) and OR products of smokers but individuals with the XRCC3 241 Thr / Thr genotype (OR, 4.13; 95% CI, 2.38-7.17). Conclusion The single nucleotide polymorphism of Thr 241 Met of XRCC3 is a predisposing factor for laryngeal and hypopharyngeal carcinoma.