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目的研究抗线粒体抗体(AMA)及其分型对原发性胆汁性肝硬化(PBC)的诊断价值。方法应用间接免疫荧光法测定血清中AMA抗体,用免疫印迹法检测AMA-M2、M4、M9亚型。78例PBC患者、35例其他肝病患者和20名健康体检者检测AMA及M2,其中30例PBC患者检测M4、M9型。结果78例PBC患者中74例(94.9%)AMA及M2均阳性。35例其他肝病患者M2均阴性。20名健康体检者AMA及M2均阴性。结论AMA及其分型,特别是M2抗体检测可作为临床诊断PBC的重要血清免疫学指标。
Objective To study the diagnostic value of anti-mitochondrial antibody (AMA) and its classification in primary biliary cirrhosis (PBC). Methods Serum AMA antibody was detected by indirect immunofluorescence method. AMA-M2, M4 and M9 subtypes were detected by immunoblotting. 78 cases of PBC patients, 35 cases of other liver disease patients and 20 healthy subjects were detected AMA and M2, of which 30 cases of PBC patients detected M4, M9 type. Results Of the 78 PBC patients, 74 (94.9%) were positive for both AMA and M2. 35 cases of other liver disease in patients with M2 were negative. 20 healthy subjects AMA and M2 were negative. Conclusion AMA and its typing, especially the detection of M2 antibodies, can be used as important serum immunological indexes for clinical diagnosis of PBC.