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【目的】了解黑龙江省大豆田大豆胞囊线虫胞囊可培养细菌的多样性。【方法】运用稀释平板法和16SrDNA基因序列的系统发育分析对胞囊可培养细菌多样性进行研究。【结果】用NA培养基从胞囊上分离90株具有不同菌落形态的细菌。16S rDNA序列分析结果表明:90株菌株分属于7个属22个种。46株属于变形菌门γ亚群(Gammaproteobacteria),32株属于厚壁菌门(Firmicutes),10株属于变形菌门β亚群(Betaproteobacteria),2株属于变形菌门ɑ亚群(Alphaproteobacteria)。假单胞菌属(Pseudomonas)和芽孢杆菌属(Bacillus)为优势菌属。【结果】黑龙江省大豆胞囊线虫胞囊中存在丰富的细菌物种多样性,这些细菌对大豆胞囊线虫可能具有一定的生理生态作用。
【Objective】 To understand the diversity of culturable bacteria of soybean cyst nematode in soybean field of Heilongjiang Province. 【Method】 The phylogenetic analysis of 16S rDNA gene sequences using dilution plate method was used to study the bacterial diversity of cysts. 【Result】 Ninety bacteria with different colony morphology were isolated from the cyst by NA medium. 16S rDNA sequence analysis showed that: 90 strains belong to 7 genera and 22 species. 46 strains belonged to Gammaproteobacteria, 32 belonged to Firmicutes, 10 belonged to Betaproteobacteria and 2 belonged to Alphaproteobacteria. Pseudomonas and Bacillus are dominant bacteria. 【Result】 The results showed that there were abundant bacterial species diversity in the cyst of soybean cyst nematode in Heilongjiang Province. These bacteria may have certain physiological and ecological effects on soybean cyst nematode.