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目的 探讨内皮素 -3 (ET -3 )及星形胶质细胞在脑缺血再灌损伤中的机制。方法 将 3 6只成年雄性昆明小鼠随机分为三组 :⑴缺血再灌组 (即IR组 ,n =2 4) ,双侧颈总动脉结扎 7min ,再灌 1、3、5、10d(每个时间点n =6)。⑵假手术组 (即ShO组 ,n =6) ,操作同上但不结扎双侧颈总动脉。⑶正常对照组 (即NG组 ,n =6)。采用免疫组化方法 ,观察脑缺血再灌小鼠额顶叶皮质ET -3和胶质纤维酸性蛋白 (GFAP)表达。结果 实验组小鼠各时间点额叶、顶叶皮质Ⅲ -Ⅵ层ET -3及GFAP免疫阳性细胞数均比对照组明显增多 (P <0 0 1) ,第 5d时 ,实验组ET -3和GFAP阳性细胞数达高峰 ,且ET -3和GFAP阳性细胞数的变化在时空上存在一致性。结论 在脑缺血再灌的早期 ,额叶、顶叶皮质ET -3和GFAP的表达显著升高 ,这可能是脑缺血再灌中枢神经功能损伤的重要机制之一。
Objective To investigate the mechanism of endothelin-3 (ET-3) and astrocyte in cerebral ischemia-reperfusion injury. Methods Thirty-six male Kunming mice were randomly divided into three groups: (1) ischemia-reperfusion group (n = 24), bilateral common carotid artery ligation for 7 min, (N = 6 at each time point). ⑵ sham operation group (ie ShO group, n = 6), the same operation but not ligation of bilateral common carotid artery. ⑶ normal control group (NG group, n = 6). Immunohistochemistry was used to observe the expression of ET-3 and glial fibrillary acidic protein (GFAP) in the frontal cortex of cerebral ischemia-reperfusion mice. Results The numbers of immunopositive cells of ET - 3 and GFAP in frontal cortex and parietal cortex in experimental group were significantly higher than those in control group at each time point (P <0.01). On the 5th day, ET - 3 And GFAP positive cells peaked, and the changes of ET-3 and GFAP-positive cells in space and time were consistent. Conclusion The expression of ET-3 and GFAP in frontal lobe and parietal cortex is significantly increased in the early stage of cerebral ischemia-reperfusion, which may be one of the important mechanisms of functional damage of central nervous system after cerebral ischemia reperfusion.