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目的:采用HPLC-DAD-ESI-MS分析芍药甘草配伍体内外物质基础变化,确认大鼠血中移行组分。方法:以Kor-masilC18色谱柱(4.6mm×250mm,5μm带保护柱);以乙腈-0.5%乙酸水为流动相进行梯度洗脱,流速0.8mL·min-1,柱温25℃,以甲醇为去蛋白溶剂,样品经电喷雾离子源正离子化后,通过LCQdeca型质谱仪,在m/z100～1000进行扫描,并对特征离子进行2次裂解,获得二级质谱数据。结果:以芍药甘草复方组方前后的体外成分研究为基础,确认了芍药甘草汤体外的主要成分为芍药苷、儿茶素-5-O-葡萄糖苷、白芍苷、甘草苷、甘草酸等,入血移行成分中也发现此类糖苷类物质,另又发现了来源于甘草成分的葡萄糖醛酸结合物——甘草素葡萄糖醛酸和异甘草酸葡萄糖醛酸。结论:采用液相色谱-质谱联用技术分析芍药甘草汤大鼠血中移行组分,并与芍药甘草复方组方前后的成分进行对比,可对复方入血的成分进行推测和确认,具有简便、快速的优点,且对于中药复方物质基础研究具有较好的应用价值。
OBJECTIVE: HPLC-DAD-ESI-MS was used to analyze the changes of the substance in vitro and in vivo of Peony licorice in vitro and in vivo. Methods: Kor-masil C18 column (4.6 mm × 250 mm, 5 μm with guard column), gradient elution with acetonitrile-0.5% acetic acid as mobile phase at a flow rate of 0.8 mL · min- As the deproteinized solvent, the sample was positively ionized by electrospray ionization, and then was scanned by LCQdeca mass spectrometer at m / z 100-1000. The characteristic ions were subjected to 2-step lysis to obtain the second-order mass spectrometry data. Results: Based on the in vitro and in vitro studies of Compound Prescriptions of Paeoniflorin and Radix Glycyrrhizae, it was confirmed that the major constituents of Paeoniflorin Soup in vitro are paeoniflorin, catechin-5-O-glucoside, white peonyone, glycyrrhizin, glycyrrhizin, , Hemorrhagic migration component also found such glycosides, and the other also found from licorice components glucuronic acid conjugates - licorice glucuronic acid and isoglycyrrhizic acid glucuronic acid. Conclusion: The composition of the blood in the rats of Shaoyao Gancao Decoction is analyzed by liquid chromatography-mass spectrometry, and compared with the ingredients before and after the compound prescription of Shaoyao Gancao, which can be used to estimate and confirm the composition of compound bleeds. , Rapid advantages, and for the basic research of traditional Chinese medicine compound has good application value.