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本工作旨在研究辣椒素对家兔房室结细胞自发活动的电生理效应及其用机制。应用经典玻璃微电极记录方法,观察到辣椒素(1-30μmol/L)剂量依赖性地抑制房室结起搏细胞的动作电位幅度,零相最大上升速度(Vmax),舒张期除极速度和起搏放电频率,而且延长复极化90％时间(APD90)。应用L型钙通道开放剂Bay K8644(0.5μtmol/L),以及提高灌流液中钙离子浓度(5 mmol/L),均可抑制辣椒素对起搏细胞的电生理效应。辣椒素受体阻断剂钌红(10 μmol/L)对辣椒素(10μmol/L)的上述电生理效应并无影响。上述结果表明,辣椒素能抑制家兔房室结的自发活动,此效应可能与其抑制钙离子内流有关,但并非由辣椒素受体介导。
This work aims to study the electrophysiological effects of capsaicin on spontaneous activity of rabbit atrioventricular node cells and its mechanism. Using classical glass microelectrode recording method, it was observed that capsaicin (1-30 μmol / L) dose-dependently inhibited the action potential amplitude, maximal zero phase velocity (Vmax), diastolic depolarization velocity Pace the discharge frequency, and extend the repolarization 90% of the time (APD90). The effects of capsaicin on the electrophysiological effects of pacemaker cells were inhibited by using Bay K8644 (0.5μtmol / L), an L-type calcium channel opener, and increasing calcium concentration in the perfusate (5mmol / L). Capsaicin receptor blocker ruthenium red (10 μmol / L) had no effect on the above electrophysiological effects of capsaicin (10 μmol / L). The above results show that capsaicin can inhibit spontaneous activity of rabbit atrioventricular node, this effect may be related to its inhibition of calcium influx, but not by the capsaicin receptor-mediated.